Cytotoxic and genotoxic effect of triphenyltin(IV) Benzylisopropyldithiocarbamate in thymoma murine cell line (WEHI 7.2)

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Abstract

This study was carried out to evaluate the cytotoxic and genotoxic effect of triphenyltin (lV)benzylisopropyldithio-carbamale compound on thymoma murine lymphoblastic leukemic cells. The MTT [3-(4,5-dimelhyllhiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay was employed for determination of cytotoxicity (IC 50) effect at different time of exposure and concentration. The ICso values obtained after treated for 24, 48 and 72 hours were 0.6 μM. The alkaline comet assay was carried out after 24 hours of treatment from MTT test and was exposed at three different time point (I, 1.5 and 2 hour) by using ICw concentration. It was found that this compound can induce DNA damage at all lime points. The average value of tail moment for three different time of exposure is 0.52 ± 0.90 A.U., 0.37 ± 0.64 A. U. and 0.46 + 0.79 A. U. DNA tail intensity is 2.55 ± 4.43 %, 1.43 ± 2.47 % and 2.54 + 4.39 %for 1, 1.5 and 2 hours of treatment. Cell morphological changes treated with IC50 values for 6, 18 and 24 hours of exposure have also been observed. In conclusion, this compound, gave cytotoxic and genotoxic effect to thymoma murine WEHI 7.2 cell at a very small dose and tends to induce cell DNA damage.

Original languageEnglish
Pages (from-to)544-549
Number of pages6
JournalResearch Journal of Chemistry and Environment
Volume15
Issue number2
Publication statusPublished - Jun 2011

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thymoma
Thymoma
DNA
Cells
cell lines
Cell Line
Assays
mice
assay
DNA damage
DNA Damage
exposure duration
inhibitory concentration 50
damage
tail
cells
Cytotoxicity
Bromides
bromide
Lime

Keywords

  • Cytotoxic
  • Dithiocarbamate
  • Genotoxic
  • Organotin
  • Thymoma
  • Triphenyltin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Earth and Planetary Sciences(all)
  • Biochemistry
  • Renewable Energy, Sustainability and the Environment
  • Chemical Engineering(all)
  • Environmental Science(all)
  • Chemistry(all)

Cite this

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title = "Cytotoxic and genotoxic effect of triphenyltin(IV) Benzylisopropyldithiocarbamate in thymoma murine cell line (WEHI 7.2)",
abstract = "This study was carried out to evaluate the cytotoxic and genotoxic effect of triphenyltin (lV)benzylisopropyldithio-carbamale compound on thymoma murine lymphoblastic leukemic cells. The MTT [3-(4,5-dimelhyllhiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay was employed for determination of cytotoxicity (IC 50) effect at different time of exposure and concentration. The ICso values obtained after treated for 24, 48 and 72 hours were 0.6 μM. The alkaline comet assay was carried out after 24 hours of treatment from MTT test and was exposed at three different time point (I, 1.5 and 2 hour) by using ICw concentration. It was found that this compound can induce DNA damage at all lime points. The average value of tail moment for three different time of exposure is 0.52 ± 0.90 A.U., 0.37 ± 0.64 A. U. and 0.46 + 0.79 A. U. DNA tail intensity is 2.55 ± 4.43 {\%}, 1.43 ± 2.47 {\%} and 2.54 + 4.39 {\%}for 1, 1.5 and 2 hours of treatment. Cell morphological changes treated with IC50 values for 6, 18 and 24 hours of exposure have also been observed. In conclusion, this compound, gave cytotoxic and genotoxic effect to thymoma murine WEHI 7.2 cell at a very small dose and tends to induce cell DNA damage.",
keywords = "Cytotoxic, Dithiocarbamate, Genotoxic, Organotin, Thymoma, Triphenyltin",
author = "Normah Awang and {Nurul Farahana}, Kamaludin and Barnard Ester and Asmah Hamid and Rajab, {Nor Fadilah} and {Abdul Halim}, Azhar",
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pages = "544--549",
journal = "Research Journal of Chemistry and Environment",
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T1 - Cytotoxic and genotoxic effect of triphenyltin(IV) Benzylisopropyldithiocarbamate in thymoma murine cell line (WEHI 7.2)

AU - Awang, Normah

AU - Nurul Farahana, Kamaludin

AU - Ester, Barnard

AU - Hamid, Asmah

AU - Rajab, Nor Fadilah

AU - Abdul Halim, Azhar

PY - 2011/6

Y1 - 2011/6

N2 - This study was carried out to evaluate the cytotoxic and genotoxic effect of triphenyltin (lV)benzylisopropyldithio-carbamale compound on thymoma murine lymphoblastic leukemic cells. The MTT [3-(4,5-dimelhyllhiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay was employed for determination of cytotoxicity (IC 50) effect at different time of exposure and concentration. The ICso values obtained after treated for 24, 48 and 72 hours were 0.6 μM. The alkaline comet assay was carried out after 24 hours of treatment from MTT test and was exposed at three different time point (I, 1.5 and 2 hour) by using ICw concentration. It was found that this compound can induce DNA damage at all lime points. The average value of tail moment for three different time of exposure is 0.52 ± 0.90 A.U., 0.37 ± 0.64 A. U. and 0.46 + 0.79 A. U. DNA tail intensity is 2.55 ± 4.43 %, 1.43 ± 2.47 % and 2.54 + 4.39 %for 1, 1.5 and 2 hours of treatment. Cell morphological changes treated with IC50 values for 6, 18 and 24 hours of exposure have also been observed. In conclusion, this compound, gave cytotoxic and genotoxic effect to thymoma murine WEHI 7.2 cell at a very small dose and tends to induce cell DNA damage.

AB - This study was carried out to evaluate the cytotoxic and genotoxic effect of triphenyltin (lV)benzylisopropyldithio-carbamale compound on thymoma murine lymphoblastic leukemic cells. The MTT [3-(4,5-dimelhyllhiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay was employed for determination of cytotoxicity (IC 50) effect at different time of exposure and concentration. The ICso values obtained after treated for 24, 48 and 72 hours were 0.6 μM. The alkaline comet assay was carried out after 24 hours of treatment from MTT test and was exposed at three different time point (I, 1.5 and 2 hour) by using ICw concentration. It was found that this compound can induce DNA damage at all lime points. The average value of tail moment for three different time of exposure is 0.52 ± 0.90 A.U., 0.37 ± 0.64 A. U. and 0.46 + 0.79 A. U. DNA tail intensity is 2.55 ± 4.43 %, 1.43 ± 2.47 % and 2.54 + 4.39 %for 1, 1.5 and 2 hours of treatment. Cell morphological changes treated with IC50 values for 6, 18 and 24 hours of exposure have also been observed. In conclusion, this compound, gave cytotoxic and genotoxic effect to thymoma murine WEHI 7.2 cell at a very small dose and tends to induce cell DNA damage.

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