Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower

Muhammad Kashif, Samina Bano, Sabira Naqvi, Shaheen Faizi, Lubna, Osman Mesaik Mohammed Ahmed Hassan, Khawaja Shamsuddin Azeemi, Ahsana Dar Farooq

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Context: Tagetes patula Linn. (Asteraceae) (French Marigold) flowers are used by local practitioners for cancer treatment; however, it lacks scientific justification. Objective: Identification of bioactive compounds in T. patula flower for cytotoxic and growth inhibition in human cancer cell lines along with its antioxidant properties using chemical and cell based systems. Materials and methods: The T. patula flower methanol extract, its seven fractions, and three phenolic compounds including methyl protocatechuate (1), patuletin (2), and patulitrin (3) were evaluated using sulforhodamine-B assay against HeLa, HT-144, NCI-H460, MCF-7, PC-3, and SF-268 human cancer cell lines. In parallel, antioxidant activity was evaluated using chemical (DPPHż, deoxyribose, and lipid peroxidation assays) and cell-based chemiluminescence systems (human neutrophils and mice macrophages). Results: The methanol extract and ethyl acetate insoluble fraction exhibited cytotoxic and growth inhibitory effects against HeLa in which 2 exhibited highest cell growth inhibition (GI50: 0.6 ± 0.1 μg/ml) and cytotoxicity (LC50: 2.5 ± 0.1 μg/ml). It also scavenged LOOż (IC50: 6.5 ± 0.7 μg/ml) and (IC50: 27.5 ± 1.3 μg/ml) in chemical systems and human neutrophils, respectively. However, 1 preferably scavenged H2O2-Cl- (IC50: 0.5 ± 0.01 μg/ml) in mice macrophages. Discussion and conclusion: Compound 2 from T. patula flower exhibited both growth inhibitory and cytotoxic properties while 1 and 3 were only growth inhibitory against HeLa. 1-3 also displayed antioxidant properties implying its probable role in growth inhibition/cytotoxic action. The present study provides scientific evidence for the use of T. patula flower in cancer treatment by traditional healer.

Original languageEnglish
Pages (from-to)672-681
Number of pages10
JournalPharmaceutical Biology
Volume53
Issue number5
DOIs
Publication statusPublished - 1 May 2015
Externally publishedYes

Fingerprint

Tagetes
Antioxidants
Growth
Inhibitory Concentration 50
lissamine rhodamine B
Methanol
Neoplasms
Neutrophils
Macrophages
Deoxyribose
Cell Line
Asteraceae
Luminescence
Lipid Peroxidation

Keywords

  • Antiproliferative
  • Free radical scavenger
  • French marigold
  • HeLa cell line
  • Patuletin

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine

Cite this

Kashif, M., Bano, S., Naqvi, S., Faizi, S., Lubna, Mohammed Ahmed Hassan, O. M., ... Farooq, A. D. (2015). Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower. Pharmaceutical Biology, 53(5), 672-681. https://doi.org/10.3109/13880209.2014.936471

Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower. / Kashif, Muhammad; Bano, Samina; Naqvi, Sabira; Faizi, Shaheen; Lubna; Mohammed Ahmed Hassan, Osman Mesaik; Azeemi, Khawaja Shamsuddin; Farooq, Ahsana Dar.

In: Pharmaceutical Biology, Vol. 53, No. 5, 01.05.2015, p. 672-681.

Research output: Contribution to journalArticle

Kashif, M, Bano, S, Naqvi, S, Faizi, S, Lubna, Mohammed Ahmed Hassan, OM, Azeemi, KS & Farooq, AD 2015, 'Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower', Pharmaceutical Biology, vol. 53, no. 5, pp. 672-681. https://doi.org/10.3109/13880209.2014.936471
Kashif M, Bano S, Naqvi S, Faizi S, Lubna, Mohammed Ahmed Hassan OM et al. Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower. Pharmaceutical Biology. 2015 May 1;53(5):672-681. https://doi.org/10.3109/13880209.2014.936471
Kashif, Muhammad ; Bano, Samina ; Naqvi, Sabira ; Faizi, Shaheen ; Lubna ; Mohammed Ahmed Hassan, Osman Mesaik ; Azeemi, Khawaja Shamsuddin ; Farooq, Ahsana Dar. / Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower. In: Pharmaceutical Biology. 2015 ; Vol. 53, No. 5. pp. 672-681.
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