Cytotoxic and antifungal activities of 5-hydroxyramulosin, a compound produced by an endophytic fungus isolated from Cinnamomum mollisimum

Carolina Santiago, Chris Fitchett, Murray H G Munro, Juriyati Jalil, Jacinta Santhanam

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22 Citations (Scopus)

Abstract

An endophytic fungus isolated from the plant Cinnamomum mollissimum was investigated for the bioactivity of its metabolites. The fungus, similar to a Phoma sp., was cultured in potato dextrose broth for two weeks, followed by extraction with ethyl acetate. The crude extract obtained was fractionated by high-performance liquid chromatography. Both crude extract and fractions were assayed for cytotoxicity against P388 murine leukemic cells and inhibition of bacterial and fungal pathogens. The bioactive extract fraction was purified further and characterized by nuclear magnetic resonance, mass spectral and X-ray crystallography analysis. A polyketide compound, 5-hydroxyramulosin, was identified as the constituent of the bioactive fungal extract fraction. This compound inhibited the fungal pathogen Aspergillus niger (IC 50 1.56μg/mL) and was cytotoxic against murine leukemia cells (IC 50 2.10μg/mL). 5-Hydroxyramulosin was the major compound produced by the endophytic fungus. This research suggests that fungal endophytes are a good source of bioactive metabolites which have potential applications in medicine.

Original languageEnglish
Article number689310
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Cinnamomum
Fungi
Complex Mixtures
Endophytes
Polyketides
Aspergillus niger
X Ray Crystallography
Solanum tuberosum
Leukemia
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
Medicine
Glucose
Research
5-hydroxyramulosin

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

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title = "Cytotoxic and antifungal activities of 5-hydroxyramulosin, a compound produced by an endophytic fungus isolated from Cinnamomum mollisimum",
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AU - Jalil, Juriyati

AU - Santhanam, Jacinta

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