Cytotoxic activity induced by crude extracts of Ganoderma lucidum (W. Curt.

Fr.) P. Karst. on mouse myeloma cancer cell-line

Chow Chin Tong, Yew Keong Choong, Nor Aini B Umar, Mohamed Mustapha Noordin, Suhaila Mohamed

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Ganoderma lucidum powder using hot water and methanol extraction methods indicated a twofold more active cytotoxic activity with IC50 of 44 ± 3.8 μg/ml in the latter method. The representative dose-response curves of the G. lucidum crude extracts on J558 cell-lines revealed that there were great similarities between the curves which reflected rapid killing activities. The percentage viability of the J558 cell exposed to these crude extracts was dose dependent only up to 150 μg/ml. After which, there was no significant reduction when the dose was increased to 200 or 400 μg/ml. The morphological alterations induced by the crude extract were examined under the phase contrast, fluorescent and electron microscopy. When J558 cells were treated with doses higher than 50 μg/ml of the crude extract, obvious morphological changes and apoptosis occurred after 72 h. At 400 μg/ml, most of the cells showed necrosis characterized as small fragments with uniformly stained red nuclei. The apoptotic and necrotic cells increased by 16.5 and 29.1%, respectively whereas the viable cells decreased by as much as 45.6. The mode of cell death via apoptosis was 3.6% higher than necrosis. However, these morphological changes were not observed in the case of 3T3 cells. Results obtained from scanning electron microscopy and transmission electron microscopy further confirmed the occurrence of various apoptotic and necrotic features.

    Original languageEnglish
    Pages (from-to)687-695
    Number of pages9
    JournalWorld Journal of Microbiology and Biotechnology
    Volume25
    Issue number4
    DOIs
    Publication statusPublished - Apr 2009

    Fingerprint

    Reishi
    Complex Mixtures
    Cell Line
    Neoplasms
    Necrosis
    Apoptosis
    Red Nucleus
    Phase-Contrast Microscopy
    3T3 Cells
    Transmission Electron Microscopy
    Electron Scanning Microscopy
    Powders
    Inhibitory Concentration 50
    Methanol
    Cell Survival
    Electron Microscopy
    Cell Death
    Water

    Keywords

    • Apoptosis
    • Ganoderma lucidum
    • IC
    • J558
    • Necrosis

    ASJC Scopus subject areas

    • Biotechnology
    • Physiology
    • Applied Microbiology and Biotechnology

    Cite this

    Cytotoxic activity induced by crude extracts of Ganoderma lucidum (W. Curt. Fr.) P. Karst. on mouse myeloma cancer cell-line. / Tong, Chow Chin; Choong, Yew Keong; Umar, Nor Aini B; Noordin, Mohamed Mustapha; Mohamed, Suhaila.

    In: World Journal of Microbiology and Biotechnology, Vol. 25, No. 4, 04.2009, p. 687-695.

    Research output: Contribution to journalArticle

    Tong, Chow Chin ; Choong, Yew Keong ; Umar, Nor Aini B ; Noordin, Mohamed Mustapha ; Mohamed, Suhaila. / Cytotoxic activity induced by crude extracts of Ganoderma lucidum (W. Curt. Fr.) P. Karst. on mouse myeloma cancer cell-line. In: World Journal of Microbiology and Biotechnology. 2009 ; Vol. 25, No. 4. pp. 687-695.
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