Cultured C2C12 cell lines as a model for assessment of bacterial attachment to bovine primary muscle cells

Siti Shahara Zulfakar, Jason D. White, Tom Ross, Mark L. Tamplin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mechanisms of bacterial attachment to meat tissues need to be understood to enhance meat safety interventions. However, little is known about attachment of foodborne pathogens to meat muscle cells. In this study, attachment of six Escherichia coli and two Salmonella strains to primary bovine muscle cells and a cultured muscle cell line, C2C12, was measured, including the effect of temperature. At 37°C, all but one strain (EC623) attached to C2C12 cells, whereas only five of eight strains (M23Sr, H10407, EC473, Sal1729a and Sal691) attached to primary cells. At 10°C, two strains (H10407 and EC473) attached to C2C12 cells, compared to four strains (M23Sr, EC614, H10407 and Sal1729a) of primary cells. Comparing all strains at both temperatures, EC614 displayed the highest CFU per C2C12 cell (4.60±2.02CFU/muscle cell at 37°C), whereas greater numbers of M23Sr attached per primary cell (51.88±39.43CFU/muscle cell at 37°C). This study indicates that primary bovine muscle cells may provide a more relevant model system to study bacterial attachment to beef carcasses compared to cell lines such as C2C12.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalMeat Science
Volume94
Issue number2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

myocytes
Muscle Cells
cultured cells
Cultured Cells
cell lines
Cell Line
cattle
Meat
meat
cells
Temperature
beef carcasses
food pathogens
Salmonella
temperature
Escherichia coli
Safety

Keywords

  • Attachment
  • CC
  • E. coli
  • Primary bovine muscle cells
  • Salmonella

ASJC Scopus subject areas

  • Food Science

Cite this

Cultured C2C12 cell lines as a model for assessment of bacterial attachment to bovine primary muscle cells. / Zulfakar, Siti Shahara; White, Jason D.; Ross, Tom; Tamplin, Mark L.

In: Meat Science, Vol. 94, No. 2, 06.2013, p. 215-219.

Research output: Contribution to journalArticle

Zulfakar, Siti Shahara ; White, Jason D. ; Ross, Tom ; Tamplin, Mark L. / Cultured C2C12 cell lines as a model for assessment of bacterial attachment to bovine primary muscle cells. In: Meat Science. 2013 ; Vol. 94, No. 2. pp. 215-219.
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