Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia

Cheng Yee Fish-Low, Ainon Hamzah, Hui Yee Chee

    Research output: Contribution to journalArticle

    Abstract

    Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence.

    Original languageEnglish
    Pages (from-to)1625-1633
    Number of pages9
    JournalSains Malaysiana
    Volume44
    Issue number11
    Publication statusPublished - 1 Nov 2015

    Fingerprint

    oil well
    microbial community
    DNA
    prokaryote
    eukaryote
    plasmid
    clone
    oil
    library
    product

    Keywords

    • 16S/18S rRNA
    • Microbial community
    • Operational taxonomic unit
    • Petroleum reservoir

    ASJC Scopus subject areas

    • General

    Cite this

    Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia. / Fish-Low, Cheng Yee; Hamzah, Ainon; Chee, Hui Yee.

    In: Sains Malaysiana, Vol. 44, No. 11, 01.11.2015, p. 1625-1633.

    Research output: Contribution to journalArticle

    Fish-Low, CY, Hamzah, A & Chee, HY 2015, 'Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia', Sains Malaysiana, vol. 44, no. 11, pp. 1625-1633.
    Fish-Low, Cheng Yee ; Hamzah, Ainon ; Chee, Hui Yee. / Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia. In: Sains Malaysiana. 2015 ; Vol. 44, No. 11. pp. 1625-1633.
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