Cultivation of intervertebral disc cells in medium fortified with growth factors improved in vitro chondrogenesis

M. Manira, B. S. Shamsul, B. S. Aminuddin, Ruszymah Idrus

Research output: Contribution to journalArticle

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Abstract

Various chondrogenic growth factors have been shown to promote proliferation, matrix production and early chondrogenesis of intervertebral disc (IVD). The objective of this study is to reconstruct IVD via tissue engineering technique and to evaluate in-vitro chon-drogenesis of chondrocytes cultured in basic growth medium with chondrogenic growth factors (FDGFs) and without chondrogenic growth factors (FD). The annulus fibrosus (AF) and nucleus pulposus (NP) were aseptically dissected from the lumbar discs of sheep and the tissues enzymatically digested and cultured in-vitro. Passage 1 cells were used to form in-vitro construct with autologous fibrin as the biomaterial. All constructs were stabilized for 14 days in their respective growth media. Histological and sulphated-glycosaminoglycan (sGAG) analysis were performed at the end of the experiment. The AF cells showed greater growth kinetic profile and higher viability in FDGFs while the NP cells showed no differences for both medium. The construct of AF and NP in FDGFs showed a bigger size construct compared to FD. The qualities of both constructs were similar as evidence by Safranin O staining in both groups. In conclusion, FDGFs increase the viability and quantity of cells for AF and not in NP. However, FDGFs supplementation improved in-vitro constructs formation for both cells type.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalBiomedical Research
Volume22
Issue number4
Publication statusPublished - Oct 2011

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Chondrogenesis
Intervertebral Disc
Intercellular Signaling Peptides and Proteins
A73025
Growth
Growth kinetics
Biocompatible Materials
Fibrin
Tissue engineering
Tissue Engineering
Chondrocytes
Tissue
Cell Survival
Sheep
Staining and Labeling
Nucleus Pulposus
Annulus Fibrosus
In Vitro Techniques
Experiments

Keywords

  • Annulus fibrosus
  • Chondro-genesis
  • Intervertebral disk
  • Nucleus pulposus
  • Sulphated-glycosaminoglycan (sGAG)
  • Tissue engineering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cultivation of intervertebral disc cells in medium fortified with growth factors improved in vitro chondrogenesis. / Manira, M.; Shamsul, B. S.; Aminuddin, B. S.; Idrus, Ruszymah.

In: Biomedical Research, Vol. 22, No. 4, 10.2011, p. 435-441.

Research output: Contribution to journalArticle

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