Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae

Nurhikmah Mohd-Sharif, Sofiyah Shaibullah, Vasanthakumar Givajothi, Cheng Seng Tan, Kok Lian Ho, Aik Hong Teh, Syarul Nataqain Baharum, Jitka Waterman, Chyan Leong Ng

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

TylP is one of five regulatory proteins involved in the regulation of antibiotic (tylosin) production, morphological and physiological differentiation in Streptomyces fradiae. Its function is similar to those of various γ butyrolactone receptor proteins. In this report, N terminally His tagged recombinant TylP protein (rTylP) was overproduced in Escherichia coli and purified to homogeneity. The rTylP protein was crystallized from a reservoir solution comprising 34%(v/v) ethylene glycol and 5%(v/v) glycerol. The protein crystals diffracted X rays to 3.05 Å resolution and belonged to the trigonal space group P3 1 21, with unit cell parameters a = b = 126.62, c = 95.63 Å.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalActa Crystallographica Section:F Structural Biology Communications
Volume73
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017

Fingerprint

Streptomyces
Crystallization
X-Rays
crystallization
proteins
X rays
Recombinant Proteins
Proteins
x rays
Tylosin
Ethylene Glycol
Glycerol
Escherichia coli
antibiotics
Escherichia
Anti-Bacterial Agents
glycerols
Crystals
homogeneity
glycols

Keywords

  • GBL
  • recombinant TylP protein
  • Streptomyces fradiae
  • transcription factors
  • tylosin
  • γ-butyrolactone

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae. / Mohd-Sharif, Nurhikmah; Shaibullah, Sofiyah; Givajothi, Vasanthakumar; Tan, Cheng Seng; Ho, Kok Lian; Teh, Aik Hong; Baharum, Syarul Nataqain; Waterman, Jitka; Ng, Chyan Leong.

In: Acta Crystallographica Section:F Structural Biology Communications, Vol. 73, No. 2, 01.02.2017, p. 109-115.

Research output: Contribution to journalArticle

Mohd-Sharif, Nurhikmah ; Shaibullah, Sofiyah ; Givajothi, Vasanthakumar ; Tan, Cheng Seng ; Ho, Kok Lian ; Teh, Aik Hong ; Baharum, Syarul Nataqain ; Waterman, Jitka ; Ng, Chyan Leong. / Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae. In: Acta Crystallographica Section:F Structural Biology Communications. 2017 ; Vol. 73, No. 2. pp. 109-115.
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