Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from Burkholderia pseudomallei

Sofiyah Shaibullah, Nurhikmah Mohd-Sharif, Kok Lian Ho, Mohd Firdaus Mohd Raih, Sheila Nathan, Rahmah Mohamed, Chyan Leong Ng

Research output: Contribution to journalArticle

Abstract

Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73Å. The calculated Matthews coefficient (V M) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%.

Original languageEnglish
Pages (from-to)1697-1700
Number of pages4
JournalActa Crystallographica Section F:Structural Biology Communications
Volume70
DOIs
Publication statusPublished - 1 Dec 2014

Fingerprint

Burkholderia pseudomallei
Crystallization
formic acid
genome
crystallization
proteins
Genes
Genome
Melioidosis
Proteins
sequencing
pathogens
DNA sequences
formates
Pathogens
infectious diseases
Escherichia
Escherichia coli
bacteria
Communicable Diseases

Keywords

  • BPSL1038
  • Burkholderia pseudomallei
  • hypothetical protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology
  • Condensed Matter Physics
  • Medicine(all)

Cite this

Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from Burkholderia pseudomallei. / Shaibullah, Sofiyah; Mohd-Sharif, Nurhikmah; Ho, Kok Lian; Mohd Raih, Mohd Firdaus; Nathan, Sheila; Mohamed, Rahmah; Ng, Chyan Leong.

In: Acta Crystallographica Section F:Structural Biology Communications, Vol. 70, 01.12.2014, p. 1697-1700.

Research output: Contribution to journalArticle

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