Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella

Nur Zazarina Ramly, Sergey N. Rouzheinikov, Svetlana E. Sedelnikova, Patrick J. Baker, Chow Yock Ping, Kiew Lian Wan, Sheila Nathan, David W. Rice

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Coccidiosis in chickens is caused by the apicomplexan parasite Eimeria tenella and is thought to involve a role for a superfamily of more than 20 cysteine-rich surface antigen glycoproteins (SAGs) in host-parasite interactions. A representative member of the family, SAG19, has been overexpressed in Escherichia coli, purified and crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. Crystals of SAG19 diffracted to beyond 1.50Å resolution and belonged to space group I4, with unit-cell parameters a = b = 108.2, c = 37.5Å. Calculation of possible values of V M suggests that there is a single molecule in the asymmetric unit.

Original languageEnglish
Pages (from-to)1380-1383
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume69
Issue number12
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Eimeria tenella
Coccidiosis
parasites
Host-Parasite Interactions
Membrane Glycoproteins
Ammonium Sulfate
antigens
Surface Antigens
Crystallization
Cysteine
Chickens
Glycoproteins
Parasites
crystallization
Escherichia coli
chickens
ammonium sulfates
cysteine
Escherichia
Vapors

Keywords

  • apicomplexa
  • Eimeria tenella
  • SAG19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella. / Ramly, Nur Zazarina; Rouzheinikov, Sergey N.; Sedelnikova, Svetlana E.; Baker, Patrick J.; Yock Ping, Chow; Wan, Kiew Lian; Nathan, Sheila; Rice, David W.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 12, 12.2013, p. 1380-1383.

Research output: Contribution to journalArticle

Ramly, Nur Zazarina ; Rouzheinikov, Sergey N. ; Sedelnikova, Svetlana E. ; Baker, Patrick J. ; Yock Ping, Chow ; Wan, Kiew Lian ; Nathan, Sheila ; Rice, David W. / Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 ; Vol. 69, No. 12. pp. 1380-1383.
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AU - Baker, Patrick J.

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