Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12

Nardiah Rizwana Jaafar, Dene Littler, Travis Beddoe, Jamie Rossjohn, Rosli Md Illias, Nor Muhammad Mahadi, Muhammad Mukram Mohamed Mackeen, Abdul Munir Abd. Murad, Farah Diba Abu Bakar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fuculose-1-phosphate aldolase (FucA) catalyses the reversible cleavage of l-fuculose 1-phosphate to dihydroxyacetone phosphate (DHAP) and l-lactaldehyde. This enzyme from mesophiles and thermophiles has been extensively studied; however, there is no report on this enzyme from a psychrophile. In this study, the gene encoding FucA from Glaciozyma antarctica PI12 (GaFucA) was cloned and the enzyme was overexpressed in Escherichia coli, purified and crystallized. The tetrameric structure of GaFucA was determined to 1.34 Å resolution. The overall architecture of GaFucA and its catalytically essential histidine triad are highly conserved among other fuculose aldolases. Comparisons of structural features between GaFucA and its mesophilic and thermophilic homologues revealed that the enzyme has typical psychrophilic attributes, indicated by the presence of a high number of nonpolar residues at the surface and a lower number of arginine residues.

Original languageEnglish
Pages (from-to)831-839
Number of pages9
JournalActa Crystallographica Section F:Structural Biology Communications
Volume72
Issue number11
DOIs
Publication statusPublished - 1 Nov 2016

Fingerprint

aldolase
Fructose-Bisphosphate Aldolase
yeast
Antarctic regions
Yeast
enzymes
phosphates
Yeasts
Crystal structure
crystal structure
Enzymes
psychrophiles
mesophiles
thermophiles
Aldehyde-Lyases
Dihydroxyacetone Phosphate
Gene encoding
histidine
Escherichia
Histidine

Keywords

  • crystallization
  • fuculose aldolase
  • Glaciozyma antarctica
  • imino cyclitols
  • l-fuculose metabolism
  • metalloenzymes
  • psychrophiles

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12. / Jaafar, Nardiah Rizwana; Littler, Dene; Beddoe, Travis; Rossjohn, Jamie; Md Illias, Rosli; Mahadi, Nor Muhammad; Mohamed Mackeen, Muhammad Mukram; Abd. Murad, Abdul Munir; Abu Bakar, Farah Diba.

In: Acta Crystallographica Section F:Structural Biology Communications, Vol. 72, No. 11, 01.11.2016, p. 831-839.

Research output: Contribution to journalArticle

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