Crystal structure of Anoxybacillus α-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass

Kian Piaw Chai, Noor Farhan Binti Othman, Aik Hong Teh, Kok Lian Ho, Kok Gan Chan, Mohd Shahir Shamsir, Kian Mau Goh, Chyan Leong Ng

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A new subfamily of glycosyl hydrolase family GH13 was recently proposed for α-amylases from Anoxybacillus species (ASKA and ADTA), Geobacillus thermoleovorans (GTA, Pizzo, and GtamyII), Bacillus aquimaris (BaqA), and 95 other putative protein homologues. To understand this new GH13 subfamily, we report crystal structures of truncated ASKA (TASKA). ASKA is a thermostable enzyme capable of producing high levels of maltose. Unlike GTA, biochemical analysis showed that Ca2+ ion supplementation enhances the catalytic activities of ASKA and TASKA. The crystal structures reveal the presence of four Ca2+ ion binding sites, with three of these binding sites are highly conserved among Anoxybacillus α-amylases. This work provides structural insights into this new GH13 subfamily both in the apo form and in complex with maltose. Furthermore, structural comparison of TASKA and GTA provides an overview of the conformational changes accompanying maltose binding at each subsite.

Original languageEnglish
Article number23126
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 15 Mar 2016

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Anoxybacillus
Maltose
Hydrolases
Amylases
Geobacillus
Binding Sites
Ions
Bacillus
Enzymes
Proteins

ASJC Scopus subject areas

  • General

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Crystal structure of Anoxybacillus α-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass. / Chai, Kian Piaw; Othman, Noor Farhan Binti; Teh, Aik Hong; Ho, Kok Lian; Chan, Kok Gan; Shamsir, Mohd Shahir; Goh, Kian Mau; Ng, Chyan Leong.

In: Scientific Reports, Vol. 6, 23126, 15.03.2016.

Research output: Contribution to journalArticle

Chai, Kian Piaw ; Othman, Noor Farhan Binti ; Teh, Aik Hong ; Ho, Kok Lian ; Chan, Kok Gan ; Shamsir, Mohd Shahir ; Goh, Kian Mau ; Ng, Chyan Leong. / Crystal structure of Anoxybacillus α-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass. In: Scientific Reports. 2016 ; Vol. 6.
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