Crystal structure and functional analysis of human C1ORF123

Siti Nurulnabila A. Rahaman, Jastina Mat Yusop, Zeti Azura Mohamed Hussein, Wan Mohd Aizat Wan Kamaruddin, Kok Lian Ho, Aik Hong Teh, Jitka Waterman, Boon Keat Tan, Hwei Ling Tan, Adelicia Yongling Li, Ee Sin Chen, Chyan Leong Ng

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Proteins of the DUF866 superfamily are exclusively found in eukaryotic cells. A member of the DUF866 superfamily, C1ORF123, is a human protein found in the open reading frame 123 of chromosome 1. The physiological role of C1ORF123 is yet to be determined. The only available protein structure of the DUF866 family shares just 26% sequence similarity and does not contain a zinc binding motif. Here, we present the crystal structure of the recombinant human C1ORF123 protein (rC1ORF123). The structure has a 2-fold internal symmetry dividing the monomeric protein into two mirrored halves that comprise of distinct electrostatic potential. The N-terminal half of rC1ORF123 includes a zinc-binding domain interacting with a zinc ion near to a potential ligand binding cavity. Functional studies of human C1ORF123 and its homologue in the fission yeast Schizosaccharomyces pombe (SpEss1) point to a role of DUF866 protein in mitochondrial oxidative phosphorylation.

Original languageEnglish
Article numbere5377
JournalPeerJ
Volume2018
Issue number9
DOIs
Publication statusPublished - 1 Jan 2018

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Functional analysis
crystal structure
Crystal structure
Zinc
Proteins
proteins
Schizosaccharomyces
Schizosaccharomyces pombe
zinc
oxidative phosphorylation
Chromosomes, Human, Pair 1
Mitochondrial Proteins
Oxidative Phosphorylation
Eukaryotic Cells
protein structure
Static Electricity
Open Reading Frames
Chromosomes
eukaryotic cells
open reading frames

Keywords

  • C1ORF123
  • Crystal structure
  • CXXC motif
  • DUF866
  • Internal symmetry
  • Mitochondrial oxidative phosphorylation
  • Zinc-binding domain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Crystal structure and functional analysis of human C1ORF123. / Rahaman, Siti Nurulnabila A.; Yusop, Jastina Mat; Mohamed Hussein, Zeti Azura; Wan Kamaruddin, Wan Mohd Aizat; Ho, Kok Lian; Teh, Aik Hong; Waterman, Jitka; Tan, Boon Keat; Tan, Hwei Ling; Li, Adelicia Yongling; Chen, Ee Sin; Ng, Chyan Leong.

In: PeerJ, Vol. 2018, No. 9, e5377, 01.01.2018.

Research output: Contribution to journalArticle

Rahaman, SNA, Yusop, JM, Mohamed Hussein, ZA, Wan Kamaruddin, WMA, Ho, KL, Teh, AH, Waterman, J, Tan, BK, Tan, HL, Li, AY, Chen, ES & Ng, CL 2018, 'Crystal structure and functional analysis of human C1ORF123', PeerJ, vol. 2018, no. 9, e5377. https://doi.org/10.7717/peerj.5377
Rahaman, Siti Nurulnabila A. ; Yusop, Jastina Mat ; Mohamed Hussein, Zeti Azura ; Wan Kamaruddin, Wan Mohd Aizat ; Ho, Kok Lian ; Teh, Aik Hong ; Waterman, Jitka ; Tan, Boon Keat ; Tan, Hwei Ling ; Li, Adelicia Yongling ; Chen, Ee Sin ; Ng, Chyan Leong. / Crystal structure and functional analysis of human C1ORF123. In: PeerJ. 2018 ; Vol. 2018, No. 9.
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