Abstract
Cryptocaryoniasis (also known as marine white spot disease) is mediated by Cryptocaryon irritans. This obligate ectoparasitic protozoan infects virtually all marine teleosts, which includes Lates calcarifer, a highly valuable aquaculture species. Little is known about L. calcarifer- C. irritans interactions. This study was undertaken to gain an informative snapshot of the L. calcarifer transcriptomic response over the course of C. irritans infection. An in-house fabricated cDNA microarray slides containing 3872 probes from L. calcarifer liver and spleen cDNA libraries were used as a tool to investigate the response of L. calcarifer to C. irritans infection. Juvenile fish were infected with parasites for four days, and total RNA was extracted from liver tissue, which was harvested daily. We compared the transcriptomes of C. irritans-infected liver to uninfected liver over an infection period of four days; the comparison was used to identify the genes with altered expression levels in response to C. irritans infection. The greatest number of infection-modulated genes was recorded at 2 and 3 days post-infection. These genes were mainly associated with the immune response and were associated in particular with the acute phase response. Acute phase proteins such as hepcidin, C-type lectin and serum amyloid A are among the highly modulated genes. Our results indicate that an induced acute phase response in L. calcarifer toward C. irritans infection is similar to the responses observed in bacterial infections of teleosts. This response demonstrates the importance of first line defenses in teleost innate immune responses against ectoparasite infection.
| Original language | English |
|---|---|
| Pages (from-to) | 788-794 |
| Number of pages | 7 |
| Journal | Fish and Shellfish Immunology |
| Volume | 33 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 2012 |
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Keywords
- Acute phase response (APR)
- CDNA microarray
- Cryptocaryon irritans
- Host-pathogen
- Lates calcarifer
ASJC Scopus subject areas
- Aquatic Science
- Environmental Chemistry
Cite this
Cryptocaryon irritans infection induces the acute phase response in Lates calcarifer : A transcriptomic perspective. / Khoo, Choon Kiat; Abd. Murad, Abdul Munir; Kua, Beng Chu; Mohd-Adnan, Adura.
In: Fish and Shellfish Immunology, Vol. 33, No. 4, 10.2012, p. 788-794.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cryptocaryon irritans infection induces the acute phase response in Lates calcarifer
T2 - A transcriptomic perspective
AU - Khoo, Choon Kiat
AU - Abd. Murad, Abdul Munir
AU - Kua, Beng Chu
AU - Mohd-Adnan, Adura
PY - 2012/10
Y1 - 2012/10
N2 - Cryptocaryoniasis (also known as marine white spot disease) is mediated by Cryptocaryon irritans. This obligate ectoparasitic protozoan infects virtually all marine teleosts, which includes Lates calcarifer, a highly valuable aquaculture species. Little is known about L. calcarifer- C. irritans interactions. This study was undertaken to gain an informative snapshot of the L. calcarifer transcriptomic response over the course of C. irritans infection. An in-house fabricated cDNA microarray slides containing 3872 probes from L. calcarifer liver and spleen cDNA libraries were used as a tool to investigate the response of L. calcarifer to C. irritans infection. Juvenile fish were infected with parasites for four days, and total RNA was extracted from liver tissue, which was harvested daily. We compared the transcriptomes of C. irritans-infected liver to uninfected liver over an infection period of four days; the comparison was used to identify the genes with altered expression levels in response to C. irritans infection. The greatest number of infection-modulated genes was recorded at 2 and 3 days post-infection. These genes were mainly associated with the immune response and were associated in particular with the acute phase response. Acute phase proteins such as hepcidin, C-type lectin and serum amyloid A are among the highly modulated genes. Our results indicate that an induced acute phase response in L. calcarifer toward C. irritans infection is similar to the responses observed in bacterial infections of teleosts. This response demonstrates the importance of first line defenses in teleost innate immune responses against ectoparasite infection.
AB - Cryptocaryoniasis (also known as marine white spot disease) is mediated by Cryptocaryon irritans. This obligate ectoparasitic protozoan infects virtually all marine teleosts, which includes Lates calcarifer, a highly valuable aquaculture species. Little is known about L. calcarifer- C. irritans interactions. This study was undertaken to gain an informative snapshot of the L. calcarifer transcriptomic response over the course of C. irritans infection. An in-house fabricated cDNA microarray slides containing 3872 probes from L. calcarifer liver and spleen cDNA libraries were used as a tool to investigate the response of L. calcarifer to C. irritans infection. Juvenile fish were infected with parasites for four days, and total RNA was extracted from liver tissue, which was harvested daily. We compared the transcriptomes of C. irritans-infected liver to uninfected liver over an infection period of four days; the comparison was used to identify the genes with altered expression levels in response to C. irritans infection. The greatest number of infection-modulated genes was recorded at 2 and 3 days post-infection. These genes were mainly associated with the immune response and were associated in particular with the acute phase response. Acute phase proteins such as hepcidin, C-type lectin and serum amyloid A are among the highly modulated genes. Our results indicate that an induced acute phase response in L. calcarifer toward C. irritans infection is similar to the responses observed in bacterial infections of teleosts. This response demonstrates the importance of first line defenses in teleost innate immune responses against ectoparasite infection.
KW - Acute phase response (APR)
KW - CDNA microarray
KW - Cryptocaryon irritans
KW - Host-pathogen
KW - Lates calcarifer
UR - http://www.scopus.com/inward/record.url?scp=84866746309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866746309&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2012.07.005
DO - 10.1016/j.fsi.2012.07.005
M3 - Article
C2 - 22842150
AN - SCOPUS:84866746309
VL - 33
SP - 788
EP - 794
JO - Fish & shellfish immunology
JF - Fish & shellfish immunology
SN - 1050-4648
IS - 4
ER -