Cryptocaryon irritans infection induces the acute phase response in Lates calcarifer: A transcriptomic perspective

Choon Kiat Khoo, Abdul Munir Abd. Murad, Beng Chu Kua, Adura Mohd-Adnan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cryptocaryoniasis (also known as marine white spot disease) is mediated by Cryptocaryon irritans. This obligate ectoparasitic protozoan infects virtually all marine teleosts, which includes Lates calcarifer, a highly valuable aquaculture species. Little is known about L. calcarifer- C. irritans interactions. This study was undertaken to gain an informative snapshot of the L. calcarifer transcriptomic response over the course of C. irritans infection. An in-house fabricated cDNA microarray slides containing 3872 probes from L. calcarifer liver and spleen cDNA libraries were used as a tool to investigate the response of L. calcarifer to C. irritans infection. Juvenile fish were infected with parasites for four days, and total RNA was extracted from liver tissue, which was harvested daily. We compared the transcriptomes of C. irritans-infected liver to uninfected liver over an infection period of four days; the comparison was used to identify the genes with altered expression levels in response to C. irritans infection. The greatest number of infection-modulated genes was recorded at 2 and 3 days post-infection. These genes were mainly associated with the immune response and were associated in particular with the acute phase response. Acute phase proteins such as hepcidin, C-type lectin and serum amyloid A are among the highly modulated genes. Our results indicate that an induced acute phase response in L. calcarifer toward C. irritans infection is similar to the responses observed in bacterial infections of teleosts. This response demonstrates the importance of first line defenses in teleost innate immune responses against ectoparasite infection.

Original languageEnglish
Pages (from-to)788-794
Number of pages7
JournalFish and Shellfish Immunology
Volume33
Issue number4
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Lates calcarifer
transcriptomics
Liver
Genes
infection
Hepcidins
C-Type Lectins
teleost
Serum Amyloid A Protein
Aquaculture
liver
Acute-Phase Proteins
Microarrays
gene
Gene Library
Fish
immune response
genes
Complementary DNA
RNA

Keywords

  • Acute phase response (APR)
  • CDNA microarray
  • Cryptocaryon irritans
  • Host-pathogen
  • Lates calcarifer

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Cryptocaryon irritans infection induces the acute phase response in Lates calcarifer : A transcriptomic perspective. / Khoo, Choon Kiat; Abd. Murad, Abdul Munir; Kua, Beng Chu; Mohd-Adnan, Adura.

In: Fish and Shellfish Immunology, Vol. 33, No. 4, 10.2012, p. 788-794.

Research output: Contribution to journalArticle

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