Cryopreservation of shoot tips of recalcitrant and tropical species

Advances and strategies

Normah Mohd. Noor, Norafarain Sulong, Barbara M. Reed

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

There is a pressing need for practical and successful conservation efforts to establish long-term germplasm collections of recalcitrant and tropical species, given the challenge and threat that these plants are facing. Cryopreservation is the only way of conserving some of these species, especially those with temperature or desiccation sensitive (recalcitrant) seeds. This review covers reports on cryopreservation studies of shoot tips (apical and axillary) of tropical and subtropical plants. Since many of these species have recalcitrant seeds, the cryopreservation successes, failures and problems involved with these seeds are also discussed. The methodologies, important factors and steps involved in successful cryopreservation protocols are analyzed. Finally strategies are suggested to develop a successful cryopreservation protocol for new plant species, in particular those with tropical recalcitrant seeds.

Original languageEnglish
JournalCryobiology
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Cryopreservation
cryopreservation
Seed
Seeds
Desiccation
germplasm conservation
Conservation
shoot tips
Temperature
seeds
recalcitrant seeds
temperature

Keywords

  • Cryopreservation
  • Desiccation-sensitive seeds
  • Freezing intolerance
  • Germplasm
  • Low temperature storage
  • Tropical plants

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Cryopreservation of shoot tips of recalcitrant and tropical species : Advances and strategies. / Mohd. Noor, Normah; Sulong, Norafarain; Reed, Barbara M.

In: Cryobiology, 01.01.2019.

Research output: Contribution to journalReview article

Mohd. Noor, Normah ; Sulong, Norafarain ; Reed, Barbara M. / Cryopreservation of shoot tips of recalcitrant and tropical species : Advances and strategies. In: Cryobiology. 2019.
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