Cryopreservation of Malayan kumquat (Fortunella polyandra) embryonic axes by vitrification

Omar Mahmoud Al Zoubi, Normah Mohd. Noor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cryopreservation of embryonic axes of Fortunella polyandra was investigated using a vitrification-based technique. Plant vitrification solution 2 (PVS2) was added to cryovials containing embryonic axes (2 mm) and kept for 0, 5, 10, 15, 20, 25 or 30 minutes at 25°C and for 0, 15, 30, 45, 60 or 75 minutes at 0°C before plunging into liquid nitrogen. Another set of embryonic axes with the same treatment but not exposed to liquid nitrogen was used as a control. The highest survival percentage of the embryonic axes was 50.0% with 15 minutes exposure to PVS2 at 25°C and 53.30% with 60 minutes exposure at 0°C. Gradual increase in exposure to PVS2 from 50% to 100% at PVS2 in one to four steps was also studied at both temperatures. The embryonic axis survival rates of 50.0-56.7% obtained for 2- to 4-step PVS2 treatments at both temperatures were significantly better than those of embryonic axes directly exposed to PVS2 (survival of 30%), showing that the period of exposure to PVS2 and sequential applications of PVS2 to the embryonic axes are important for successful cryopreservation of F. polyandra.

Original languageEnglish
Pages (from-to)758-762
Number of pages5
JournalAustralian Journal of Crop Science
Volume7
Issue number6
Publication statusPublished - May 2013

Fingerprint

Fortunella
vitrification
cryopreservation
liquids
nitrogen
exposure duration
temperature
survival rate

Keywords

  • Cryopreservation
  • Embryonic axis
  • Fortunella polyandra
  • Vitrification

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Cryopreservation of Malayan kumquat (Fortunella polyandra) embryonic axes by vitrification. / Al Zoubi, Omar Mahmoud; Mohd. Noor, Normah.

In: Australian Journal of Crop Science, Vol. 7, No. 6, 05.2013, p. 758-762.

Research output: Contribution to journalArticle

Al Zoubi, Omar Mahmoud ; Mohd. Noor, Normah. / Cryopreservation of Malayan kumquat (Fortunella polyandra) embryonic axes by vitrification. In: Australian Journal of Crop Science. 2013 ; Vol. 7, No. 6. pp. 758-762.
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