Cryopreservation of excised embryos and embryonic axes

Normah Mohd. Noor, A. M. Makeen

Research output: Chapter in Book/Report/Conference proceedingChapter

36 Citations (Scopus)

Abstract

Seeds are categorized into two main groups according to their response to desiccation and their storage physiology: orthodox (desiccation-tolerant) and recalcitrant (desiccation-sensitive) seeds (Roberts 1973). A third category of seeds are those that are relatively desiccation tolerant but do not withstand desiccation down to water contents as low as those tolerated by orthodox seeds. These seeds are freezing sensitive and are referred to as intermediate seeds (Ellis et al. 1990, 1991). Seed is the most preferred plant propagule for ex situ germplasm conservation due to low storage cost, ease of seed handling and regeneration of whole plants from genetically diverse materials (Chin 1994; Pritchard 1995). While orthodox seeds are acquiescent to storage under conventional gene bank conditions for centuries, (i.e. 3-7% seed water content at -20°C) (FAO/IPGRI 1994), cryopreservation is the only available option for longterm storage of non-orthodox seeds. However, in circumstances where storage of the whole seed of a non-orthodox species is constrained by desiccation and freezing sensitivity on one hand, and by its relatively large seeds on the other, excised embryos and embryonic axes are an alternative option. In other cases where viability of lipid-rich orthodox seeds under conventional storage conditions is drastically reduced due to the thin seed coat coupled with lipid peroxidation, as in the case of peanuts (Arachis hypogaea), germplasm curators can resort to cryopreservation of the excised embryonic axes (Gagliardi et al. 2002). Several studies on recalcitrant and intermediate species empirically determined that excised embryos and embryonic axes (in most cases) are more tolerant to desiccation and subsequent cryoexposure than whole seeds (e.g. Bajaj 1984; Radhamani and Chandel 1992; Normah et al. 1994; Makeen et al. 2005)

Original languageEnglish
Title of host publicationPlant Cryopreservation: A Practical Guide
PublisherSpringer New York
Pages211-240
Number of pages30
ISBN (Print)9780387722764, 9780387722757
DOIs
Publication statusPublished - 2008

Fingerprint

Cryopreservation
embryo (plant)
cryopreservation
Seed
Seeds
Embryonic Structures
Desiccation
seeds
Freezing
Water content
freezing
water content
Lipids
gene banks
Chin
Water
germplasm conservation
Arachis hypogaea
Physiology
storage conditions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mohd. Noor, N., & Makeen, A. M. (2008). Cryopreservation of excised embryos and embryonic axes. In Plant Cryopreservation: A Practical Guide (pp. 211-240). Springer New York. https://doi.org/10.1007/978-0-387-72276-4_10

Cryopreservation of excised embryos and embryonic axes. / Mohd. Noor, Normah; Makeen, A. M.

Plant Cryopreservation: A Practical Guide. Springer New York, 2008. p. 211-240.

Research output: Chapter in Book/Report/Conference proceedingChapter

Mohd. Noor, N & Makeen, AM 2008, Cryopreservation of excised embryos and embryonic axes. in Plant Cryopreservation: A Practical Guide. Springer New York, pp. 211-240. https://doi.org/10.1007/978-0-387-72276-4_10
Mohd. Noor N, Makeen AM. Cryopreservation of excised embryos and embryonic axes. In Plant Cryopreservation: A Practical Guide. Springer New York. 2008. p. 211-240 https://doi.org/10.1007/978-0-387-72276-4_10
Mohd. Noor, Normah ; Makeen, A. M. / Cryopreservation of excised embryos and embryonic axes. Plant Cryopreservation: A Practical Guide. Springer New York, 2008. pp. 211-240
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