Cross-platform expression profiling demonstrates that SV40 small tumor antigen activates Notch, Hedgehog, and Wnt signaling in human cells

Mohamed Ali-Seyed, Noelani Laycock, Suresh Karanam, Wenming Xiao, Eric T. Blair, Carlos S. Moreno

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: We previously analyzed human embryonic kidney (HEK) cell lines for the effects that simian virus 40 (SV40) small tumor antigen (ST) has on gene expression using Affymetrix U133 GeneChips. To cross-validate and extend our initial findings, we sought to compare the expression profiles of these cell lines using an alternative microarray platform. Methods: We have analyzed matched cell lines with and without expression of SV40 ST using an Applied Biosystems (AB) microarray platform that uses single 60-mer oligonucleotides and single-color quantitative chemiluminescence for detection. Results: While we were able to previously identify only 456 genes affected by ST with the Affymetrix platform, we identified 1927 individual genes with the AB platform. Additional technical replicates increased the number of identified genes to 3478 genes and confirmed the changes in 278 (61%) of our original set of 456 genes. Among the 3200 genes newly identified as affected by SV40 ST, we confirmed 20 by QRTPCR including several components of the Wnt, Notch, and Hedgehog signaling pathways, consistent with SV40 ST activation of these developmental pathways. While inhibitors of Notch activation had no effect on cell survival, cyclopamine had a potent killing effect on cells expressing SV40 ST. Conclusions: These data show that SV40 ST expression alters cell survival pathways to sensitize cells to the killing effect of Hedgehog pathway inhibitors.

Original languageEnglish
Article number54
JournalBMC Cancer
Volume6
DOIs
Publication statusPublished - 7 Mar 2006
Externally publishedYes

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Simian virus 40
Neoplasm Antigens
Genes
Cell Line
Cell Survival
Luminescence
Oligonucleotides
Color
Kidney
Gene Expression

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Genetics

Cite this

Cross-platform expression profiling demonstrates that SV40 small tumor antigen activates Notch, Hedgehog, and Wnt signaling in human cells. / Ali-Seyed, Mohamed; Laycock, Noelani; Karanam, Suresh; Xiao, Wenming; Blair, Eric T.; Moreno, Carlos S.

In: BMC Cancer, Vol. 6, 54, 07.03.2006.

Research output: Contribution to journalArticle

Ali-Seyed, Mohamed ; Laycock, Noelani ; Karanam, Suresh ; Xiao, Wenming ; Blair, Eric T. ; Moreno, Carlos S. / Cross-platform expression profiling demonstrates that SV40 small tumor antigen activates Notch, Hedgehog, and Wnt signaling in human cells. In: BMC Cancer. 2006 ; Vol. 6.
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AU - Blair, Eric T.

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