Cosmos caudatus enhances fracture healing in ovariectomised rats: A preliminary biomechanical evaluation

Pamela Rufus Godspower, Norazlina Mohamed, Ahmad Nazrun Shuid

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2 Citations (Scopus)

Abstract

Osteoporotic fractures occur in osteoporotic states and affect patients' quality of life. Cosmos caudatus (ulam raja) is a local plant known for its high calcium content and anti-oxidant properties. The present study aimed to investigate the fracture healing properties of C. caudatus water extract in ovariectomised rats by studying the biomechanical properties of tibia. Twenty-four female Sprague-Dawley rats were divided into 4 groups: (i) sham operated (ii) ovariectomised control (iii) ovariectomised + estrogen (100μg/kg/day) and (iv) ovariectomised + C. caudatus (500mg/kg). Following six weeks of sham operation or ovariectomy, the right tibia of the rats were fractured. Rats were then given their respective treatment for 8 weeks with body weight monitored weekly. Biomechanical analysis indicated that the maximum load, stress and Young's modulus of the ovariectomised control group (36.2 ± 4.7N, 10.01 ± 1.41MPa, 29.2 ± 9.39MPa respectively) were significantly lower compared to sham operated (150.32 ± 32. 6N, 36.75 ± 7.98MPa, 183 ± 53.2MPa respectively) and the C. caudatus treated group (136.86 ± 16.95N, 33.45 ± 4.14MPa, 155.13± 58.58MPa respectively). Therefore, C. caudatus extract improved the biomechanical property of the healed bone and may be beneficial for fracture healing in the estrogen deficient state. Industrial Relevance: Post-menopausal osteoporosis is a debilitating disease affecting women worldwide. Hormone replacement therapy (HRT), commonly used for the prevention and treatment of post-menopausal osteoporosis has been associated with several side effects. Thus, in finding alternatives in the treatment of osteoporosis, C. caudatus is a plant of interest. Previous study showed that C. caudatus improved bone histomorphometry in ovariectomized rats by increasing double-labeled surface (dLS/BS), mineral appositional rate (MAR), osteoid volume (OV/BV) and osteoblast surface (Ob.S/BS). Therefore, the present study aimed to assess the fracture healing properties of C. caudatus extract. The results of this study may offer a potential use of C. caudatus in the healing of fractures in the osteoporotic state.

Original languageEnglish
Pages (from-to)12-19
Number of pages8
JournalInternational Journal of Applied Research in Natural Products
Volume8
Issue number1
Publication statusPublished - 2015

Fingerprint

Fracture Healing
Postmenopausal Osteoporosis
Osteoporotic Fractures
Tibia
Estrogens
Skates (Fish)
Bone and Bones
Elastic Modulus
Hormone Replacement Therapy
Ovariectomy
Osteoblasts
Oxidants
Osteoporosis
Minerals
Sprague Dawley Rats
Therapeutics
Body Weight
Quality of Life
Calcium
Control Groups

Keywords

  • Biomechanics
  • Cosmos caudatus
  • Fracture healing
  • Osteoporosis
  • Ovariectomy

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Complementary and alternative medicine

Cite this

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title = "Cosmos caudatus enhances fracture healing in ovariectomised rats: A preliminary biomechanical evaluation",
abstract = "Osteoporotic fractures occur in osteoporotic states and affect patients' quality of life. Cosmos caudatus (ulam raja) is a local plant known for its high calcium content and anti-oxidant properties. The present study aimed to investigate the fracture healing properties of C. caudatus water extract in ovariectomised rats by studying the biomechanical properties of tibia. Twenty-four female Sprague-Dawley rats were divided into 4 groups: (i) sham operated (ii) ovariectomised control (iii) ovariectomised + estrogen (100μg/kg/day) and (iv) ovariectomised + C. caudatus (500mg/kg). Following six weeks of sham operation or ovariectomy, the right tibia of the rats were fractured. Rats were then given their respective treatment for 8 weeks with body weight monitored weekly. Biomechanical analysis indicated that the maximum load, stress and Young's modulus of the ovariectomised control group (36.2 ± 4.7N, 10.01 ± 1.41MPa, 29.2 ± 9.39MPa respectively) were significantly lower compared to sham operated (150.32 ± 32. 6N, 36.75 ± 7.98MPa, 183 ± 53.2MPa respectively) and the C. caudatus treated group (136.86 ± 16.95N, 33.45 ± 4.14MPa, 155.13± 58.58MPa respectively). Therefore, C. caudatus extract improved the biomechanical property of the healed bone and may be beneficial for fracture healing in the estrogen deficient state. Industrial Relevance: Post-menopausal osteoporosis is a debilitating disease affecting women worldwide. Hormone replacement therapy (HRT), commonly used for the prevention and treatment of post-menopausal osteoporosis has been associated with several side effects. Thus, in finding alternatives in the treatment of osteoporosis, C. caudatus is a plant of interest. Previous study showed that C. caudatus improved bone histomorphometry in ovariectomized rats by increasing double-labeled surface (dLS/BS), mineral appositional rate (MAR), osteoid volume (OV/BV) and osteoblast surface (Ob.S/BS). Therefore, the present study aimed to assess the fracture healing properties of C. caudatus extract. The results of this study may offer a potential use of C. caudatus in the healing of fractures in the osteoporotic state.",
keywords = "Biomechanics, Cosmos caudatus, Fracture healing, Osteoporosis, Ovariectomy",
author = "Godspower, {Pamela Rufus} and Norazlina Mohamed and Shuid, {Ahmad Nazrun}",
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T1 - Cosmos caudatus enhances fracture healing in ovariectomised rats

T2 - A preliminary biomechanical evaluation

AU - Godspower, Pamela Rufus

AU - Mohamed, Norazlina

AU - Shuid, Ahmad Nazrun

PY - 2015

Y1 - 2015

N2 - Osteoporotic fractures occur in osteoporotic states and affect patients' quality of life. Cosmos caudatus (ulam raja) is a local plant known for its high calcium content and anti-oxidant properties. The present study aimed to investigate the fracture healing properties of C. caudatus water extract in ovariectomised rats by studying the biomechanical properties of tibia. Twenty-four female Sprague-Dawley rats were divided into 4 groups: (i) sham operated (ii) ovariectomised control (iii) ovariectomised + estrogen (100μg/kg/day) and (iv) ovariectomised + C. caudatus (500mg/kg). Following six weeks of sham operation or ovariectomy, the right tibia of the rats were fractured. Rats were then given their respective treatment for 8 weeks with body weight monitored weekly. Biomechanical analysis indicated that the maximum load, stress and Young's modulus of the ovariectomised control group (36.2 ± 4.7N, 10.01 ± 1.41MPa, 29.2 ± 9.39MPa respectively) were significantly lower compared to sham operated (150.32 ± 32. 6N, 36.75 ± 7.98MPa, 183 ± 53.2MPa respectively) and the C. caudatus treated group (136.86 ± 16.95N, 33.45 ± 4.14MPa, 155.13± 58.58MPa respectively). Therefore, C. caudatus extract improved the biomechanical property of the healed bone and may be beneficial for fracture healing in the estrogen deficient state. Industrial Relevance: Post-menopausal osteoporosis is a debilitating disease affecting women worldwide. Hormone replacement therapy (HRT), commonly used for the prevention and treatment of post-menopausal osteoporosis has been associated with several side effects. Thus, in finding alternatives in the treatment of osteoporosis, C. caudatus is a plant of interest. Previous study showed that C. caudatus improved bone histomorphometry in ovariectomized rats by increasing double-labeled surface (dLS/BS), mineral appositional rate (MAR), osteoid volume (OV/BV) and osteoblast surface (Ob.S/BS). Therefore, the present study aimed to assess the fracture healing properties of C. caudatus extract. The results of this study may offer a potential use of C. caudatus in the healing of fractures in the osteoporotic state.

AB - Osteoporotic fractures occur in osteoporotic states and affect patients' quality of life. Cosmos caudatus (ulam raja) is a local plant known for its high calcium content and anti-oxidant properties. The present study aimed to investigate the fracture healing properties of C. caudatus water extract in ovariectomised rats by studying the biomechanical properties of tibia. Twenty-four female Sprague-Dawley rats were divided into 4 groups: (i) sham operated (ii) ovariectomised control (iii) ovariectomised + estrogen (100μg/kg/day) and (iv) ovariectomised + C. caudatus (500mg/kg). Following six weeks of sham operation or ovariectomy, the right tibia of the rats were fractured. Rats were then given their respective treatment for 8 weeks with body weight monitored weekly. Biomechanical analysis indicated that the maximum load, stress and Young's modulus of the ovariectomised control group (36.2 ± 4.7N, 10.01 ± 1.41MPa, 29.2 ± 9.39MPa respectively) were significantly lower compared to sham operated (150.32 ± 32. 6N, 36.75 ± 7.98MPa, 183 ± 53.2MPa respectively) and the C. caudatus treated group (136.86 ± 16.95N, 33.45 ± 4.14MPa, 155.13± 58.58MPa respectively). Therefore, C. caudatus extract improved the biomechanical property of the healed bone and may be beneficial for fracture healing in the estrogen deficient state. Industrial Relevance: Post-menopausal osteoporosis is a debilitating disease affecting women worldwide. Hormone replacement therapy (HRT), commonly used for the prevention and treatment of post-menopausal osteoporosis has been associated with several side effects. Thus, in finding alternatives in the treatment of osteoporosis, C. caudatus is a plant of interest. Previous study showed that C. caudatus improved bone histomorphometry in ovariectomized rats by increasing double-labeled surface (dLS/BS), mineral appositional rate (MAR), osteoid volume (OV/BV) and osteoblast surface (Ob.S/BS). Therefore, the present study aimed to assess the fracture healing properties of C. caudatus extract. The results of this study may offer a potential use of C. caudatus in the healing of fractures in the osteoporotic state.

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