Correlative transmission electron microscopy and scanning electron microscopy of rabbit cornea

O. Fauziah, M. Siti-Saleha, Norzana Abd. Ghafar, R. Asmah, S. Aminuddin, Ruszymah Idrus

Research output: Contribution to journalArticle

Abstract

The present work was carried out to study the organization of rabbit cornea. The transparent nature of the normal cornea was very important to give the understanding of the cornea in health and disease state. The ultrastructural features of the cornea were obtained by transmission electron microscopy and scanning electron microscopy. The epithelial surface of the cornea composed of microvilli and microplicae. In the Bowman's membrane, the individual collagen fibrils were interwoven densely to form a felt-like sheet, while in the stroma, the collagen fibrils ran abreast in lamellae. In the innermost stromal region adjacent to Descemet's membrane, collagen fibrils were oriented in various directions and interlaced, forming loose fibrillar networks. The endothelial layer is a single cell layer and apically-localized below the Descemet's membrane. This present work will be a standard reference for future research pertaining to cornea particularly cornea tissue engineering.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalMalaysian Journal of Microscopy
Volume4
Issue number1
Publication statusPublished - 2010

Fingerprint

cornea
rabbits
Transmission Electron Microscopy
Collagen
Electron Scanning Microscopy
Cornea
Rabbits
Transmission electron microscopy
Membranes
transmission electron microscopy
Scanning electron microscopy
scanning electron microscopy
collagens
Descemet Membrane
Tissue engineering
membranes
Cells
Health
Bowman Membrane
tissue engineering

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Instrumentation

Cite this

Correlative transmission electron microscopy and scanning electron microscopy of rabbit cornea. / Fauziah, O.; Siti-Saleha, M.; Abd. Ghafar, Norzana; Asmah, R.; Aminuddin, S.; Idrus, Ruszymah.

In: Malaysian Journal of Microscopy, Vol. 4, No. 1, 2010, p. 50-57.

Research output: Contribution to journalArticle

@article{077c1eea850f464d85f22dca43ca88dc,
title = "Correlative transmission electron microscopy and scanning electron microscopy of rabbit cornea",
abstract = "The present work was carried out to study the organization of rabbit cornea. The transparent nature of the normal cornea was very important to give the understanding of the cornea in health and disease state. The ultrastructural features of the cornea were obtained by transmission electron microscopy and scanning electron microscopy. The epithelial surface of the cornea composed of microvilli and microplicae. In the Bowman's membrane, the individual collagen fibrils were interwoven densely to form a felt-like sheet, while in the stroma, the collagen fibrils ran abreast in lamellae. In the innermost stromal region adjacent to Descemet's membrane, collagen fibrils were oriented in various directions and interlaced, forming loose fibrillar networks. The endothelial layer is a single cell layer and apically-localized below the Descemet's membrane. This present work will be a standard reference for future research pertaining to cornea particularly cornea tissue engineering.",
author = "O. Fauziah and M. Siti-Saleha and {Abd. Ghafar}, Norzana and R. Asmah and S. Aminuddin and Ruszymah Idrus",
year = "2010",
language = "English",
volume = "4",
pages = "50--57",
journal = "Malaysian Journal of Microscopy",
issn = "1823-7010",
publisher = "Universiti Putra Malaysia",
number = "1",

}

TY - JOUR

T1 - Correlative transmission electron microscopy and scanning electron microscopy of rabbit cornea

AU - Fauziah, O.

AU - Siti-Saleha, M.

AU - Abd. Ghafar, Norzana

AU - Asmah, R.

AU - Aminuddin, S.

AU - Idrus, Ruszymah

PY - 2010

Y1 - 2010

N2 - The present work was carried out to study the organization of rabbit cornea. The transparent nature of the normal cornea was very important to give the understanding of the cornea in health and disease state. The ultrastructural features of the cornea were obtained by transmission electron microscopy and scanning electron microscopy. The epithelial surface of the cornea composed of microvilli and microplicae. In the Bowman's membrane, the individual collagen fibrils were interwoven densely to form a felt-like sheet, while in the stroma, the collagen fibrils ran abreast in lamellae. In the innermost stromal region adjacent to Descemet's membrane, collagen fibrils were oriented in various directions and interlaced, forming loose fibrillar networks. The endothelial layer is a single cell layer and apically-localized below the Descemet's membrane. This present work will be a standard reference for future research pertaining to cornea particularly cornea tissue engineering.

AB - The present work was carried out to study the organization of rabbit cornea. The transparent nature of the normal cornea was very important to give the understanding of the cornea in health and disease state. The ultrastructural features of the cornea were obtained by transmission electron microscopy and scanning electron microscopy. The epithelial surface of the cornea composed of microvilli and microplicae. In the Bowman's membrane, the individual collagen fibrils were interwoven densely to form a felt-like sheet, while in the stroma, the collagen fibrils ran abreast in lamellae. In the innermost stromal region adjacent to Descemet's membrane, collagen fibrils were oriented in various directions and interlaced, forming loose fibrillar networks. The endothelial layer is a single cell layer and apically-localized below the Descemet's membrane. This present work will be a standard reference for future research pertaining to cornea particularly cornea tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=78049493482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049493482&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:78049493482

VL - 4

SP - 50

EP - 57

JO - Malaysian Journal of Microscopy

JF - Malaysian Journal of Microscopy

SN - 1823-7010

IS - 1

ER -