Correlation of microspore nuclear development with male inflorescence morphology in Elaeis oleifera, Elaeis guineensis and the OxG hybrid

A. M. Latif, N. M. Zaki, H. M N Nordiana, M. Marhalil, Che Radziah Che Mohd. Zain, M. Madon

Research output: Contribution to journalArticle

Abstract

Microspore nuclear stages of the Elaeis species and their OxG hybrid were examined and correlated with the respective male inflorescence morphology. Isolated pollen mother cells (PMC) and microspores obtained from squashed male flower buds were stained with blue fluorescent 4',6-diamidino-2-phenylindole (DAPI) dye and observed with the Zeiss Axioplan epifluorescent microscope to determine their nuclear stages. The male inflorescence from both the Elaeis species and the (OxG) hybrid with a perfectly closed spathe, having whitish inflorescences and friable individual spikelets indicated that PMC activities were ongoing within the anthers of male flower buds. In E. guineensis, the whitish, pale yellow or light green spikelets with flexible texture obtained from a perfectly closed spathe appeared to be morphological markers for uninucleate microspores. For E. oleifera inflorescences, the colour varies from pale beige to umber brown with flexible spikelets, while the spathe varied from being perfectly closed to slightly open. The observed morphology indicated the presence of uninucleate microspores in E. oleifera. For the OxG hybrid morphological markers resembled that of E. oleifera. By staining a plethora of male flower buds from various oil palm male inflorescences, the meiosis and mitosis phases were also profiled.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalJournal of Oil Palm Research
Volume26
Issue numberMAR
Publication statusPublished - 2014

Fingerprint

Elaeis oleifera
Inflorescence
Elaeis guineensis
microspores
inflorescences
Methyl Green
Palm oil
male flowers
Microscopes
Coloring Agents
Elaeis
Dyes
Textures
Pollen
Color
buds
Stem Cells
Meiosis
pollen
Mitosis

Keywords

  • Male inflorescence
  • Microspore
  • Nuclear development

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Biomaterials
  • Agronomy and Crop Science
  • Food Science

Cite this

Correlation of microspore nuclear development with male inflorescence morphology in Elaeis oleifera, Elaeis guineensis and the OxG hybrid. / Latif, A. M.; Zaki, N. M.; Nordiana, H. M N; Marhalil, M.; Che Mohd. Zain, Che Radziah; Madon, M.

In: Journal of Oil Palm Research, Vol. 26, No. MAR, 2014, p. 104-108.

Research output: Contribution to journalArticle

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