Correlation between the number of anaerobic bacteria in the sediment determined by MPN method and organics of the overlying water in the Jungnang-cheon

Doo Hyun Park, Byung Hong Kim, Si Keun Lim, Young Hyo Choi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sediments collected from the Jungnang-cheon and its tributaries were used to enumerate anaerobic bacteria by most probable number (MPN) methods. A simple method was developed to detect ferrous ion in the culture fluid in order to count the number of iron ion reducers, and sulfate-reducing bacteria (SRB) and methanogens were detected by the presence of FeS precipitate in the culture or methane in the head space, respectively. The numbers of iron reducer was in the range of 107∼108 /g in the sediment of the stream containing higher organic content than the tributaries. The sediments of tributaries were analyzed to contain iron reducers less than 107 cells/g. With one exception the numbers of SRB and methanogens were less than 103 cells/g in the sediment. From these results it is concluded that organics in the sediment support the growth of iron reducers, which out-compete SRB and methanogens.

Original languageEnglish
Pages (from-to)364-370
Number of pages7
JournalKorean Journal of Applied Microbiology and Biotechnology
Volume24
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Anaerobic Bacteria
Iron
Sulfates
Water
Bacteria
Ions
Methane
Head
Growth

Keywords

  • Metal reducing bacteria
  • Methanogen
  • Most probable number
  • SRB

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Correlation between the number of anaerobic bacteria in the sediment determined by MPN method and organics of the overlying water in the Jungnang-cheon. / Park, Doo Hyun; Kim, Byung Hong; Lim, Si Keun; Choi, Young Hyo.

In: Korean Journal of Applied Microbiology and Biotechnology, Vol. 24, No. 3, 1996, p. 364-370.

Research output: Contribution to journalArticle

@article{338a0bfeee5f46a5a1f624582e2eb43b,
title = "Correlation between the number of anaerobic bacteria in the sediment determined by MPN method and organics of the overlying water in the Jungnang-cheon",
abstract = "Sediments collected from the Jungnang-cheon and its tributaries were used to enumerate anaerobic bacteria by most probable number (MPN) methods. A simple method was developed to detect ferrous ion in the culture fluid in order to count the number of iron ion reducers, and sulfate-reducing bacteria (SRB) and methanogens were detected by the presence of FeS precipitate in the culture or methane in the head space, respectively. The numbers of iron reducer was in the range of 107∼108 /g in the sediment of the stream containing higher organic content than the tributaries. The sediments of tributaries were analyzed to contain iron reducers less than 107 cells/g. With one exception the numbers of SRB and methanogens were less than 103 cells/g in the sediment. From these results it is concluded that organics in the sediment support the growth of iron reducers, which out-compete SRB and methanogens.",
keywords = "Metal reducing bacteria, Methanogen, Most probable number, SRB",
author = "Park, {Doo Hyun} and Kim, {Byung Hong} and Lim, {Si Keun} and Choi, {Young Hyo}",
year = "1996",
language = "English",
volume = "24",
pages = "364--370",
journal = "Microbiology and Biotechnology Letters",
issn = "1598-642X",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "3",

}

TY - JOUR

T1 - Correlation between the number of anaerobic bacteria in the sediment determined by MPN method and organics of the overlying water in the Jungnang-cheon

AU - Park, Doo Hyun

AU - Kim, Byung Hong

AU - Lim, Si Keun

AU - Choi, Young Hyo

PY - 1996

Y1 - 1996

N2 - Sediments collected from the Jungnang-cheon and its tributaries were used to enumerate anaerobic bacteria by most probable number (MPN) methods. A simple method was developed to detect ferrous ion in the culture fluid in order to count the number of iron ion reducers, and sulfate-reducing bacteria (SRB) and methanogens were detected by the presence of FeS precipitate in the culture or methane in the head space, respectively. The numbers of iron reducer was in the range of 107∼108 /g in the sediment of the stream containing higher organic content than the tributaries. The sediments of tributaries were analyzed to contain iron reducers less than 107 cells/g. With one exception the numbers of SRB and methanogens were less than 103 cells/g in the sediment. From these results it is concluded that organics in the sediment support the growth of iron reducers, which out-compete SRB and methanogens.

AB - Sediments collected from the Jungnang-cheon and its tributaries were used to enumerate anaerobic bacteria by most probable number (MPN) methods. A simple method was developed to detect ferrous ion in the culture fluid in order to count the number of iron ion reducers, and sulfate-reducing bacteria (SRB) and methanogens were detected by the presence of FeS precipitate in the culture or methane in the head space, respectively. The numbers of iron reducer was in the range of 107∼108 /g in the sediment of the stream containing higher organic content than the tributaries. The sediments of tributaries were analyzed to contain iron reducers less than 107 cells/g. With one exception the numbers of SRB and methanogens were less than 103 cells/g in the sediment. From these results it is concluded that organics in the sediment support the growth of iron reducers, which out-compete SRB and methanogens.

KW - Metal reducing bacteria

KW - Methanogen

KW - Most probable number

KW - SRB

UR - http://www.scopus.com/inward/record.url?scp=6544231180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6544231180&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:6544231180

VL - 24

SP - 364

EP - 370

JO - Microbiology and Biotechnology Letters

JF - Microbiology and Biotechnology Letters

SN - 1598-642X

IS - 3

ER -