Abstract
Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52% ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.
| Original language | English |
|---|---|
| Article number | 485 |
| Journal | Polymers |
| Volume | 9 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 3 Oct 2017 |
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Keywords
- Controlled release
- Double-walled microspheres
- Drug delivery
- Poly(lactide-coglycolide)
- Therapeutic proteins
ASJC Scopus subject areas
- Chemistry(all)
- Polymers and Plastics
Cite this
Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. / Ansary, Rezaul H.; Rahman, Mokhlesur M.; Mohamad, Nasir; Arrif, Tengku M.; Latif, Ahmad Zubaidi A.; Katas, Haliza; Wan Nik, Wan Sani B.; Awang, Mohamed B.
In: Polymers, Vol. 9, No. 10, 485, 03.10.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
AU - Ansary, Rezaul H.
AU - Rahman, Mokhlesur M.
AU - Mohamad, Nasir
AU - Arrif, Tengku M.
AU - Latif, Ahmad Zubaidi A.
AU - Katas, Haliza
AU - Wan Nik, Wan Sani B.
AU - Awang, Mohamed B.
PY - 2017/10/3
Y1 - 2017/10/3
N2 - Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52% ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.
AB - Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52% ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.
KW - Controlled release
KW - Double-walled microspheres
KW - Drug delivery
KW - Poly(lactide-coglycolide)
KW - Therapeutic proteins
UR - http://www.scopus.com/inward/record.url?scp=85030539169&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030539169&partnerID=8YFLogxK
U2 - 10.3390/polym9100485
DO - 10.3390/polym9100485
M3 - Article
VL - 9
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 10
M1 - 485
ER -