Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres

Rezaul H. Ansary, Mokhlesur M. Rahman, Nasir Mohamad, Tengku M. Arrif, Ahmad Zubaidi A. Latif, Haliza Katas, Wan Sani B. Wan Nik, Mohamed B. Awang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52% ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.

Original languageEnglish
Article number485
JournalPolymers
Volume9
Issue number10
DOIs
Publication statusPublished - 3 Oct 2017

Fingerprint

Polyglactin 910
Muramidase
Microspheres
Enzymes
Polyvinyl Alcohol
Trehalose
Polyvinyl alcohols
Excipients
Structural integrity
Encapsulation
Oils
Proteins
Emulsification
Water
Bioactivity
polylactic acid-polyglycolic acid copolymer
Emulsions
Hydroxyl Radical
Peptides
Glucose

Keywords

  • Controlled release
  • Double-walled microspheres
  • Drug delivery
  • Poly(lactide-coglycolide)
  • Therapeutic proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Cite this

Ansary, R. H., Rahman, M. M., Mohamad, N., Arrif, T. M., Latif, A. Z. A., Katas, H., ... Awang, M. B. (2017). Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. Polymers, 9(10), [485]. https://doi.org/10.3390/polym9100485

Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. / Ansary, Rezaul H.; Rahman, Mokhlesur M.; Mohamad, Nasir; Arrif, Tengku M.; Latif, Ahmad Zubaidi A.; Katas, Haliza; Wan Nik, Wan Sani B.; Awang, Mohamed B.

In: Polymers, Vol. 9, No. 10, 485, 03.10.2017.

Research output: Contribution to journalArticle

Ansary, RH, Rahman, MM, Mohamad, N, Arrif, TM, Latif, AZA, Katas, H, Wan Nik, WSB & Awang, MB 2017, 'Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres', Polymers, vol. 9, no. 10, 485. https://doi.org/10.3390/polym9100485
Ansary, Rezaul H. ; Rahman, Mokhlesur M. ; Mohamad, Nasir ; Arrif, Tengku M. ; Latif, Ahmad Zubaidi A. ; Katas, Haliza ; Wan Nik, Wan Sani B. ; Awang, Mohamed B. / Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. In: Polymers. 2017 ; Vol. 9, No. 10.
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abstract = "Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52{\%} ± 3.28{\%}) was achieved when 1{\%} (w/v) polyvinyl alcohol (PVA) and 2.5{\%} (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.",
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AU - Arrif, Tengku M.

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AU - Katas, Haliza

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