Control of ganoderma boninense

A causal agent of basal stem rot disease in oil palm with endophyte bacteria in vitro

M. Rahamath Bivi, M. Sitinoor Farhana, A. Khairulmazmi, Idris Abd. Ghani

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The objective of this study was to screen the potential endophytic bacteria to be used as a biological control for Ganoderma boninense, the major causal pathogen of Basal Stem Rot (BSR) disease in oil palm. Twenty endophytic bacteria were isolated from symptomless oil palm roots but only seven isolates showed inhibitory effect by suppressing the mycelial growth of G. boninense. The isolated bacteria were screened in vitro by dual culture assay and culture filtrate test for their antagonistic properties towards G. boninense. Four bacterial endophytes (EB2, EB4, EB5 & EB6) were established to have potential as biocontrol agents based on their percentage inhibition of radial growth (PIRG) more than 50%. There was significantly difference in inhibitory effect (p<0.05) for dual culture test and these endophytic bacteria inhibited the fungal growth by an average of 52.78, 83.33, 67.59 and 93.52%, respectively on NA medium. Culture filtrate test likewise was showed a significant different for percentage of mycelia growth (p<0.05). Endophytic bacteria EB2, EB4, EB5 and EB6 suppressed the fungal growth by an average of 56.65, 91.45, 69.48 and 97.95%, respectively. Interestingly, out of four potential endophytic bacterial isolates, two, isolates namely EB4 and EB6, produced significantly (p<0.05) higher inhibitory effect than two more isolates of EB2 and EB5. Changes of hyphae occurred in presence of both bacteria of EB4 and EB6. Deformity and shrinkage of hyphae in EB6 was more apparent than in EB4 through mycelial growth test. Identification of species for these two bacteria was done using Biolog® System where EB6 was successfully identified as Pseudomonas aeruginosa but EB4 could not be unidentified. Therefore, in vitro activities of EB4 and P. aeruginosa against G. boninense in these studies suggested that these endophytic bacteria can be used as an effective biological control agent.

Original languageEnglish
Pages (from-to)833-839
Number of pages7
JournalInternational Journal of Agriculture and Biology
Volume12
Issue number6
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Ganoderma
Endophytes
stem rot
Elaeis guineensis
endophytes
Oils
Bacteria
bacteria
Growth
Hyphae
culture filtrates
Pseudomonas aeruginosa
hyphae
biological control agents
microbial growth
testing
Biological Control Agents
Ganoderma boninense
In Vitro Techniques
Mycelium

Keywords

  • Basal stem rot disease
  • Culture filtrate test
  • Dual culture assay test
  • Mycelia test
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Control of ganoderma boninense : A causal agent of basal stem rot disease in oil palm with endophyte bacteria in vitro. / Bivi, M. Rahamath; Farhana, M. Sitinoor; Khairulmazmi, A.; Abd. Ghani, Idris.

In: International Journal of Agriculture and Biology, Vol. 12, No. 6, 2010, p. 833-839.

Research output: Contribution to journalArticle

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