Continuous Butanol Fermentation of Dilute Acid-Pretreated De-oiled Rice Bran by Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani, Abdualati Ibrahim Al-Tabib, Abudukeremu Kadier, Mohd Fauzi Zanil, Kiat Moon Lee, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

Abstract

Continuous fermentation of dilute acid-pretreated de-oiled rice bran (DRB) to butanol by the Clostridium acetobutylicum YM1 strain was investigated. Pretreatment of DRB with dilute sulfuric acid (1%) resulted in the production of 42.12 g/L total sugars, including 25.57 g/L glucose, 15.1 g/L xylose and 1.46 g/L cellobiose. Pretreated-DRB (SADRB) was used as a fermentation medium at various dilution rates, and a dilution rate of 0.02 h −1 was optimal for solvent production, in which 11.18 g/L of total solvent was produced (acetone 4.37 g/L, butanol 5.89 g/L and ethanol 0.92 g/L). Detoxification of SADRB with activated charcoal resulted in the high removal of fermentation inhibitory compounds. Fermentation of detoxified-SADRB in continuous fermentation with a dilution rate of 0.02 h −1 achieved higher concentrations of solvent (12.42 g/L) and butanol (6.87 g/L), respectively, with a solvent productivity of 0.248 g/L.h. This study showed that the solvent concentration and productivity in continuous fermentation from SADRB was higher than that obtained from batch culture fermentation. This study also provides an economic assessment for butanol production in continuous fermentation process from DRB to validate the commercial viability of this process.

Original languageEnglish
Article number4622
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Clostridium acetobutylicum
Butanols
Fermentation
Acids
Cellobiose
Batch Cell Culture Techniques
Oryza
Xylose
Charcoal
Acetone
Ethanol
Economics
Glucose

ASJC Scopus subject areas

  • General

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Continuous Butanol Fermentation of Dilute Acid-Pretreated De-oiled Rice Bran by Clostridium acetobutylicum YM1. / Al-Shorgani, Najeeb Kaid Nasser; Al-Tabib, Abdualati Ibrahim; Kadier, Abudukeremu; Zanil, Mohd Fauzi; Lee, Kiat Moon; Kalil, Mohd. Sahaid.

In: Scientific Reports, Vol. 9, No. 1, 4622, 01.12.2019.

Research output: Contribution to journalArticle

Al-Shorgani, Najeeb Kaid Nasser ; Al-Tabib, Abdualati Ibrahim ; Kadier, Abudukeremu ; Zanil, Mohd Fauzi ; Lee, Kiat Moon ; Kalil, Mohd. Sahaid. / Continuous Butanol Fermentation of Dilute Acid-Pretreated De-oiled Rice Bran by Clostridium acetobutylicum YM1. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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