Consumption of heated palm oil and its effect on kidney in rats

Kamsiah Jaarin, Chan Pee Yeen, Norliana Masbah

Research output: Contribution to journalArticle

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Abstract

This research studied the effects of heated palm oil on serum creatinine and kidney histological changes in rats. Forty female Sprague-Dawley rats were divided into four groups (I to IV). Group I was fed with normal rats chaw (control). The groups II, III and IV were fed with rat chaw fortified with 15% weight/weight (w/w) fresh palm oil (FPO), heated five times palm oil (5HPO) and heated ten times palm oil (10HPO), respectively for a period of 16 weeks. Blood for serum creatinine was taken and determined at baseline and at the end of the study period using commercial kit and Cobas integra analyzer. The rats were sacrificed and the kidneys were weighed and examined histologically. The tissues were stained with haematoxylin and eosin. There was a significant reduction in the kidney's weight in 5HPO and 10HPO group compared to fresh and control group. At the end of 16 weeks there was a significant increase in serum creatinine in all study groups compared to the respective baseline. However, there was no significant difference in serum creatinine in the palm oil fed groups compared with control. Histologically, localized congestion was noted in the tubular region of the kidney in the group fed with FPO, while diffuse congestion was observed in the glomerulus and tubular region of the group fed with 5HPO and 10HPO. There was also evidence of chronic inflammation with lymphocytes infiltration located in the tubular region of the groups fed with 5HPO and 10HPO. The inflammatory changes were more severe in 10HPO compared to 5HPO group. In conclusion; heated palm oil diet reduced kidney's weight, causes tubular congestion and inflammation which were more severe in 10HPO compared to 5HPO. The histological changes were not accompanied by an increase in serum creatinine level.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalPakistan Journal of Nutrition
Volume15
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

palm oils
kidneys
blood serum
creatinine
Kidney
Creatinine
rats
Weights and Measures
Serum
inflammation
Inflammation
Hematoxylin
Eosine Yellowish-(YS)
palm oil
Sprague Dawley Rats
lymphocytes
Lymphocytes
Diet
Control Groups
Research

Keywords

  • Heated
  • Histology
  • Inflammation
  • Kidney
  • Palm oil

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics
  • Public Health, Environmental and Occupational Health

Cite this

Consumption of heated palm oil and its effect on kidney in rats. / Jaarin, Kamsiah; Yeen, Chan Pee; Masbah, Norliana.

In: Pakistan Journal of Nutrition, Vol. 15, No. 2, 2016, p. 148-154.

Research output: Contribution to journalArticle

Jaarin, Kamsiah ; Yeen, Chan Pee ; Masbah, Norliana. / Consumption of heated palm oil and its effect on kidney in rats. In: Pakistan Journal of Nutrition. 2016 ; Vol. 15, No. 2. pp. 148-154.
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