Construction of plant expression vector harbouring Oak 1 gene encoding kalata B1 protein

Norsharina Md Saad, Teo Chee How, Zuraida Ab Rahman, Zamri Zainal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The prototypic cyclotide (Kalata B1), isolated from African plant Oldenlandia affinis (Rubiceae), exhibits molluscicidal property against golden apple snails. Given their naturally high mollucicidal activities against golden apple snail, and reports on the introduction of the Oak 1 gene into various plant species, there is a possibility to generate transgenic rice that is able to produce cyclotides protein. In this study, the full-length Oak 1 gene was synthesized and amplified using two specific oligonucleotide primers. The Oak 1 gene was cloned into the pCAMBIA1301 vector using a conventional molecular cloning method. The expression of the Oak 1 gene will be driven by strong constitutive Zea mays Ubiquitin (UBI) promoter. This construct can be transformed into rice callus through Agrobacterium-mediated system to produce transgenic rice.

Original languageEnglish
Title of host publication2018 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium
EditorsNoor Hayati Ahmad Rasol, Kamarulzaman Ibrahim, Siti Aishah Hasbullah, Mohammad Hafizuddin Hj. Jumali, Nazlina Ibrahim, Marlia Mohd Hanafiah, Mohd Talib Latif
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418431
DOIs
Publication statusPublished - 27 Jun 2019
Event2018 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 4 Apr 20186 Apr 2018

Publication series

NameAIP Conference Proceedings
Volume2111
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2018 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period4/4/186/4/18

Fingerprint

genes
Quercus
coding
rice
snails
Pomacea canaliculata
proteins
protein
gene
snail
molluscicidal properties
Oldenlandia
genetically modified organisms
primers
corn
oligonucleotides
DNA primers
ubiquitin
Agrobacterium
molecular cloning

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

Cite this

Saad, N. M., How, T. C., Rahman, Z. A., & Zainal, Z. (2019). Construction of plant expression vector harbouring Oak 1 gene encoding kalata B1 protein. In N. H. A. Rasol, K. Ibrahim, S. A. Hasbullah, M. H. H. Jumali, N. Ibrahim, M. M. Hanafiah, & M. T. Latif (Eds.), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium [040001] (AIP Conference Proceedings; Vol. 2111). American Institute of Physics Inc.. https://doi.org/10.1063/1.5111240

Construction of plant expression vector harbouring Oak 1 gene encoding kalata B1 protein. / Saad, Norsharina Md; How, Teo Chee; Rahman, Zuraida Ab; Zainal, Zamri.

2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. ed. / Noor Hayati Ahmad Rasol; Kamarulzaman Ibrahim; Siti Aishah Hasbullah; Mohammad Hafizuddin Hj. Jumali; Nazlina Ibrahim; Marlia Mohd Hanafiah; Mohd Talib Latif. American Institute of Physics Inc., 2019. 040001 (AIP Conference Proceedings; Vol. 2111).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Saad, NM, How, TC, Rahman, ZA & Zainal, Z 2019, Construction of plant expression vector harbouring Oak 1 gene encoding kalata B1 protein. in NHA Rasol, K Ibrahim, SA Hasbullah, MHH Jumali, N Ibrahim, MM Hanafiah & MT Latif (eds), 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium., 040001, AIP Conference Proceedings, vol. 2111, American Institute of Physics Inc., 2018 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 4/4/18. https://doi.org/10.1063/1.5111240
Saad NM, How TC, Rahman ZA, Zainal Z. Construction of plant expression vector harbouring Oak 1 gene encoding kalata B1 protein. In Rasol NHA, Ibrahim K, Hasbullah SA, Jumali MHH, Ibrahim N, Hanafiah MM, Latif MT, editors, 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. American Institute of Physics Inc. 2019. 040001. (AIP Conference Proceedings). https://doi.org/10.1063/1.5111240
Saad, Norsharina Md ; How, Teo Chee ; Rahman, Zuraida Ab ; Zainal, Zamri. / Construction of plant expression vector harbouring Oak 1 gene encoding kalata B1 protein. 2018 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2018 Postgraduate Colloquium. editor / Noor Hayati Ahmad Rasol ; Kamarulzaman Ibrahim ; Siti Aishah Hasbullah ; Mohammad Hafizuddin Hj. Jumali ; Nazlina Ibrahim ; Marlia Mohd Hanafiah ; Mohd Talib Latif. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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