Construction of bacterial artificial chromosome library from electrochemical microorganisms

Jung Ho Back, Man Su Kim, Hyuk Cho, In Seop Chang, Jiyoung Lee, Kyung Sik Kim, Byung Hong Kim, Young In Park, Ye Sun Han

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A microbial fuel cell is a device that directly converts metabolic energy into electricity, using electrochemical technology. The analysis of large genome fragments recovered directly from microbial communities represents one promising approach to characterizing uncultivated electrochemical microorganisms. To further assess the utility of this approach, we constructed large-insert (140 kb) bacterial artificial chromosome (BAC) libraries from the genomic DNA of a microbial fuel cell, which had been operated for three weeks using acetate media. We screened the expression of several ferric reductase activities in the Escherichia coli host, in order to determine the extent of heterologous expression of metal-ion-reducing enzymes in the library. Phylogenetic analysis of 16S rRNA gene sequences recovered from the BAC libraries indicates that they contain DNA from a wide diversity of microbial organisms. The constructed bacterial library proved a powerful tool for exploring metal-ion reductase activities, providing information on the electron transport pathway of electrochemical microbial (ECM) organisms.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalFEMS Microbiology Letters
Volume238
Issue number1
DOIs
Publication statusPublished - 1 Sep 2004
Externally publishedYes

Fingerprint

Bioelectric Energy Sources
Bacterial Artificial Chromosomes
Libraries
Metals
Ions
Electricity
Genomic Library
Electron Transport
Gene Library
rRNA Genes
Oxidoreductases
Acetates
Genome
Escherichia coli
Technology
Equipment and Supplies
DNA
Enzymes

Keywords

  • Bacterial artificial chromosome library
  • Electrochemical microbial organisms
  • Metal reductase
  • Microbial fuel cell

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Back, J. H., Kim, M. S., Cho, H., Chang, I. S., Lee, J., Kim, K. S., ... Han, Y. S. (2004). Construction of bacterial artificial chromosome library from electrochemical microorganisms. FEMS Microbiology Letters, 238(1), 65-70. https://doi.org/10.1016/j.femsle.2004.07.018

Construction of bacterial artificial chromosome library from electrochemical microorganisms. / Back, Jung Ho; Kim, Man Su; Cho, Hyuk; Chang, In Seop; Lee, Jiyoung; Kim, Kyung Sik; Kim, Byung Hong; Park, Young In; Han, Ye Sun.

In: FEMS Microbiology Letters, Vol. 238, No. 1, 01.09.2004, p. 65-70.

Research output: Contribution to journalArticle

Back, JH, Kim, MS, Cho, H, Chang, IS, Lee, J, Kim, KS, Kim, BH, Park, YI & Han, YS 2004, 'Construction of bacterial artificial chromosome library from electrochemical microorganisms', FEMS Microbiology Letters, vol. 238, no. 1, pp. 65-70. https://doi.org/10.1016/j.femsle.2004.07.018
Back, Jung Ho ; Kim, Man Su ; Cho, Hyuk ; Chang, In Seop ; Lee, Jiyoung ; Kim, Kyung Sik ; Kim, Byung Hong ; Park, Young In ; Han, Ye Sun. / Construction of bacterial artificial chromosome library from electrochemical microorganisms. In: FEMS Microbiology Letters. 2004 ; Vol. 238, No. 1. pp. 65-70.
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