Construction of a Hydrogel Pectin-Based Triglyceride Optical Biosensor with Immobilized Lipase Enzymes

Uswatun Hasanah, Nor Diyana Md Sani, Lee Yook Heng, Rinaldi Idroes, Eka Safitri

Research output: Contribution to journalArticle

Abstract

A novel and simple optical biosensor to detect triglycerides (TGs) has been successfully constructed by using pectin hydrogel membrane as the indicator pH and chromoionophore ETH 5294 (CI), with lipase as the catalyst. The enzymatic working system against TGs releasing H+ ions will affect the color absorbance of CI. The characterization results show that a TG biosensor has the optimum condition and sensitivity at the phosphate buffer concentration of 50 mM, pH 7, and enzyme loading of 60 μg. The biosensor works at the tripalmitin (TP) concentration range of 100-400 mg/dL. With the sensitivity of 0.001 (∆A/(mg/dL)), the biosensor response reaches stability after five minutes, and the limit of detection (LOD) of the TG optical biosensor is 15 mg/dL. Relative standard deviation (RSD) in a reproducibility test was 2.5%, with a 15-day lifespan.

Original languageEnglish
JournalBiosensors
Volume9
Issue number4
DOIs
Publication statusPublished - 13 Nov 2019

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Immobilized Enzymes
Hydrogel
Biosensing Techniques
Lipase
Biosensors
Triglycerides
Enzymes
Limit of Detection
Buffers
Color
Phosphates
pectin
Ions
Membranes
Catalysts

Keywords

  • chromoionophore
  • pectin
  • triglyceride optical biosensor

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Construction of a Hydrogel Pectin-Based Triglyceride Optical Biosensor with Immobilized Lipase Enzymes. / Hasanah, Uswatun; Sani, Nor Diyana Md; Heng, Lee Yook; Idroes, Rinaldi; Safitri, Eka.

In: Biosensors, Vol. 9, No. 4, 13.11.2019.

Research output: Contribution to journalArticle

Hasanah, Uswatun ; Sani, Nor Diyana Md ; Heng, Lee Yook ; Idroes, Rinaldi ; Safitri, Eka. / Construction of a Hydrogel Pectin-Based Triglyceride Optical Biosensor with Immobilized Lipase Enzymes. In: Biosensors. 2019 ; Vol. 9, No. 4.
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