Komposisi minyak pati rizom Hedychium malayanum dan aktiviti antipengoksidanya

Translated title of the contribution: Composition of Hedychium malayanum rhizome essential oil and its antioxidant activity

Mohammed Yahya Abdo, Wan Yaacob Wan Ahmad, Laily Bin Din, Nazlina Ibrahim

Research output: Contribution to journalArticle

Abstract

The Hedychium malayanum rhizome oil with a yield of 0.079% was obtained after hydro distillation using a Clevenger-type apparatus. Nineteen compounds representing 99.5% of the total amount of oil were identified using capillary GC and GC-MS with a DB-5 column. The oil was dominated by monoterpenes (99.0%) with 1,8-cineole (37.7%), β-pinene (35.2%) and α-pinene (10.9%) being the most abundant constituents. The oil was studied for its antioxidant activities by three different tests, involving its effect on the 1,1-diphenyl-2-picrylhydrazil (radical scavenging activity, RSA), ferrous ion chelating (FIC) and β-carotene bleaching (BCB) in linoleic acid system. The oil showed fairly good activities toward all the above tests compared to the standards except in the case of FIC, where the standard ascorbic acid possessed weaker chelating ability than the oil. RSA was calculated as IC50, and the respective values for gallic acid, ascorbic acid and the oil were 1.8 ± 0.06, 3.5 ± 0.30 and 140.3 ± 1.46 µg/mL. For BCB, the oil and standard BHT had possessed high and very high capacity (87.2 ± 1.00 and 96.8 ± 0.21% respectively) to prevent the oxidation by linoleic acid. FIC activity showed that the oil and standard ascorbic acid were weaker chelating than EDTA (IC50 86.9 ± 0.70 µg/mL). However, the oil showed a fairly good chelating (IC50 283.3 ± 5.26 µg/mL) whereas the ascorbic acid possessed a weak activity to chelate Fe2+ (IC50 1428.6 ± 7.04 µg/mL).

Original languageMalay
Pages (from-to)1147-1155
Number of pages9
JournalMalaysian Journal of Analytical Sciences
Volume19
Issue number6
Publication statusPublished - 2015

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Volatile Oils
Oils
Antioxidants
Chelation
Chemical analysis
Ascorbic Acid
Scavenging
Linoleic Acid
Ions
Butylated Hydroxytoluene
Gallic Acid
Monoterpenes
Carotenoids
Bleaching
Edetic Acid
Distillation
Thermodynamic properties
Oxidation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Komposisi minyak pati rizom Hedychium malayanum dan aktiviti antipengoksidanya. / Yahya Abdo, Mohammed; Wan Ahmad, Wan Yaacob; Din, Laily Bin; Ibrahim, Nazlina.

In: Malaysian Journal of Analytical Sciences, Vol. 19, No. 6, 2015, p. 1147-1155.

Research output: Contribution to journalArticle

Yahya Abdo, Mohammed ; Wan Ahmad, Wan Yaacob ; Din, Laily Bin ; Ibrahim, Nazlina. / Komposisi minyak pati rizom Hedychium malayanum dan aktiviti antipengoksidanya. In: Malaysian Journal of Analytical Sciences. 2015 ; Vol. 19, No. 6. pp. 1147-1155.
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AU - Ibrahim, Nazlina

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AB - The Hedychium malayanum rhizome oil with a yield of 0.079% was obtained after hydro distillation using a Clevenger-type apparatus. Nineteen compounds representing 99.5% of the total amount of oil were identified using capillary GC and GC-MS with a DB-5 column. The oil was dominated by monoterpenes (99.0%) with 1,8-cineole (37.7%), β-pinene (35.2%) and α-pinene (10.9%) being the most abundant constituents. The oil was studied for its antioxidant activities by three different tests, involving its effect on the 1,1-diphenyl-2-picrylhydrazil (radical scavenging activity, RSA), ferrous ion chelating (FIC) and β-carotene bleaching (BCB) in linoleic acid system. The oil showed fairly good activities toward all the above tests compared to the standards except in the case of FIC, where the standard ascorbic acid possessed weaker chelating ability than the oil. RSA was calculated as IC50, and the respective values for gallic acid, ascorbic acid and the oil were 1.8 ± 0.06, 3.5 ± 0.30 and 140.3 ± 1.46 µg/mL. For BCB, the oil and standard BHT had possessed high and very high capacity (87.2 ± 1.00 and 96.8 ± 0.21% respectively) to prevent the oxidation by linoleic acid. FIC activity showed that the oil and standard ascorbic acid were weaker chelating than EDTA (IC50 86.9 ± 0.70 µg/mL). However, the oil showed a fairly good chelating (IC50 283.3 ± 5.26 µg/mL) whereas the ascorbic acid possessed a weak activity to chelate Fe2+ (IC50 1428.6 ± 7.04 µg/mL).

KW - Antioxidant activity

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KW - GC-GC/MS

KW - Hedychium malayanum

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