Comparison of the effects of α-tocopherol and γ-tocotrienol against oxidative stress in two different neuronal cultures

Sue Mian Then, Wan Zurinah Wan Ngah, Gapor Mat Top, Musalmah Mazlan

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Tocopherols and tocotrienols liave been sliown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2 and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF) in their neuroprotective effects against H202-induced apoptosis in primary rat cortical neurons and human neuroblastoma cell line SH-SY5Y. Cytotoxicity screening of H 2O2, GTT and ATF was done to determine the IC50 levels. To screen for neuroprotective effects, cortical neurons and SH-SY5Y cell cultures were pre-incubated with GTT or ATF, respectively at different concentrations for 1 hour before concurrent treatment Of H2O 2 at IC30. Results of these treatments were compared to cells treated with H2O2 only and control cells. Cytotoxicology screening showed that IC50 of H2O 2 for cortical neuron is at 50 μM while SH-SY5Y have higher IC50 of 100 μM. GTT is cytotoxic to cortical neurons at ≥50 μM and SH-SY5Yat≥l00 μ M while ATF did not show any toxicity within the range of concentration tested (1-750 μM). Results from neuroprotection screening showed that GTT and ATF were able to protect both cortical neurons and SH-SY5Yfrom H2O2-induced oxidative stress at concetration of≤10 μCellular uptake of GTT is higher in both cortical neurons and SH-SY5Y as compared to ATF when both cortical neuron and SH-SY5Y were incubated with 10 μ M GTT or ATF, respectively for 24 hour. Although primary rat cortical neurons and human neuroblastoma SH-SY5Y were different culture system, the effects of GTT and ATF are similar in both H2O2 -induced culture which strongly suggest that both GTT and ATF act as free radical scavenger to exert their neuroprotective effects.

    Original languageEnglish
    Pages (from-to)145-156
    Number of pages12
    JournalSains Malaysiana
    Volume39
    Issue number1
    Publication statusPublished - Feb 2010

    Fingerprint

    Tocotrienols
    Tocopherols
    Oxidative Stress
    Neurons
    Neuroprotective Agents
    Inhibitory Concentration 50
    Neuroblastoma
    Buthionine Sulfoximine
    Free Radical Scavengers
    Vitamin E
    Hydrogen Peroxide
    Cell Culture Techniques
    Apoptosis

    Keywords

    • Apoptosis
    • Neurons
    • Oxidative stress
    • Tocopherol
    • Tocotrienol

    ASJC Scopus subject areas

    • General

    Cite this

    Comparison of the effects of α-tocopherol and γ-tocotrienol against oxidative stress in two different neuronal cultures. / Then, Sue Mian; Wan Ngah, Wan Zurinah; Top, Gapor Mat; Mazlan, Musalmah.

    In: Sains Malaysiana, Vol. 39, No. 1, 02.2010, p. 145-156.

    Research output: Contribution to journalArticle

    Then, Sue Mian ; Wan Ngah, Wan Zurinah ; Top, Gapor Mat ; Mazlan, Musalmah. / Comparison of the effects of α-tocopherol and γ-tocotrienol against oxidative stress in two different neuronal cultures. In: Sains Malaysiana. 2010 ; Vol. 39, No. 1. pp. 145-156.
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