Comparison of telomere length and telomerase activation between breast fibroadenoma and infiltrating ductal carcinoma in Malaysian women

Lai Meng Looi, Phaik Leng Cheah, Min Hwei Ng, Cheng Har Yip, Kein Seong Mun, Nazarina Abdul Rahman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A study was initiated to explore possible differences in handling telomere attrition in the most common malignant and benign tumours of the breast in Malaysian women. Infiltrating ductal carcinoma (IDC) and fibroadenoma (FA) represented the malignant and benign prototypes respectively. 29 IDC, 28 FA and 22 benign non-lesional control (BNL) breast tissue samples were analysed for telomerase activation using a Telomerase PCR ELISA kit (Boehringer Mannheim). In addition, 23 IDC, 12 FA and 14 BNL were subjected to telomere length determination with a TeloTAGGG Telomere Length Assay Kit (Roche Diagnostic GmbH, Germany), following digestion of genomic DNA by frequently cutting restriction enzymes RsaI and HinfI. Mean telomerase activity in IDC (A450nm=0.3338), but not FA (A450nm=0.0003) was significantly raised (p<0.05) compared with BNL (A450nm=0.0031). Similarly IDC (1.2 kb), but not FA (2.2 kb), showed significant telomere shortening (p<0.05) relative to BNL (2.9 kb). The findings imply that telomere attrition and telomerase activation differ between malignant and benign tumours of the breast and may be important for targeted therapy.

Original languageEnglish
Pages (from-to)713-716
Number of pages4
JournalAsian Pacific Journal of Cancer Prevention
Volume11
Issue number3
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Fibroadenoma
Ductal Carcinoma
Telomerase
Telomere
Breast
Breast Neoplasms
Telomere Shortening
Germany
Digestion
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
DNA
Enzymes

Keywords

  • Breast
  • Fibroadenoma
  • Infiltrating ductal carcinoma
  • Telomerase
  • Telomere length

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology

Cite this

Comparison of telomere length and telomerase activation between breast fibroadenoma and infiltrating ductal carcinoma in Malaysian women. / Looi, Lai Meng; Cheah, Phaik Leng; Ng, Min Hwei; Yip, Cheng Har; Mun, Kein Seong; Rahman, Nazarina Abdul.

In: Asian Pacific Journal of Cancer Prevention, Vol. 11, No. 3, 2010, p. 713-716.

Research output: Contribution to journalArticle

Looi, Lai Meng ; Cheah, Phaik Leng ; Ng, Min Hwei ; Yip, Cheng Har ; Mun, Kein Seong ; Rahman, Nazarina Abdul. / Comparison of telomere length and telomerase activation between breast fibroadenoma and infiltrating ductal carcinoma in Malaysian women. In: Asian Pacific Journal of Cancer Prevention. 2010 ; Vol. 11, No. 3. pp. 713-716.
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