Comparison of physicochemical analysis and antioxidant activities of Nigella sativa seeds and oils from Yemen, Iran and Malaysia

Hasnah Haron, Chong Grace-Lynn, Suzana Shahar

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11 Citations (Scopus)

Abstract

The study was aimed to analyze the physicochemical properties and antioxidant activities in five batches of seeds and oils of Nigella sativa, obtained from Malaysia, Iran and Yemen. Proximate analysis showed that the seeds contained 20.63-28.71% crude fat, 11.35-14.04% crude protein, 5.37-7.93% total moisture, 4.15-4.51% total ash contents and 48.69-57.18% total carbohydrate contents. Physicochemical analysis showed a refractive index of 1.4697-1.4730, specific gravity of 1.369-1.376 g/cm3, peroxide value of 3.33-21.33 meq O2/kg, 184-220 mg/g in saponification number and unsaponifiable matter of 1.1-1.8% in the oil samples. The seeds showed high mineral content such as Ca (2242 mg/kg), K (6393 mg/kg) and Mg (2234 mg/kg). The oil sample from Kelantan, Malaysia contained the lowest saturated fatty acid (SFA) (1.42±0.29%) while Sudan, Yemen contained the highest content of polyunsaturated fatty acid (PUFA) (65.13±5.45%). Monounsaturated fatty acid (MUFA) were found the highest (20.45±2.61%) in the seed samples originated from Iran. Seeds from Iran showed the highest antioxidant activity (IC50 = 1.49 mg/mL) and total phenolic content (30.84 mg GAE/g) while oil sample from Sudan, Yemen has the highest antioxidant activity (IC 50 = 4.48 mg/mL). Seeds from Iran have the highest quality among the seed samples while oil samples from Kelantan, Malaysia was the best among the oil samples in terms of low SFA, high PUFA, MUFA and antioxidant activities.

Original languageEnglish
Pages (from-to)535-542
Number of pages8
JournalSains Malaysiana
Volume43
Issue number4
Publication statusPublished - 2014

Fingerprint

Nigella sativa
Yemen
Malaysia
Iran
antioxidant activity
oils
seeds
sampling
Sudan
monounsaturated fatty acids
saturated fatty acids
inhibitory concentration 50
polyunsaturated fatty acids
saponification number
refractive index
peroxide value
seed quality
specific gravity
proximate composition
carbohydrate content

Keywords

  • Antioxidants
  • Nigella sativa
  • Oil
  • Physicochemical
  • Seeds

ASJC Scopus subject areas

  • General

Cite this

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title = "Comparison of physicochemical analysis and antioxidant activities of Nigella sativa seeds and oils from Yemen, Iran and Malaysia",
abstract = "The study was aimed to analyze the physicochemical properties and antioxidant activities in five batches of seeds and oils of Nigella sativa, obtained from Malaysia, Iran and Yemen. Proximate analysis showed that the seeds contained 20.63-28.71{\%} crude fat, 11.35-14.04{\%} crude protein, 5.37-7.93{\%} total moisture, 4.15-4.51{\%} total ash contents and 48.69-57.18{\%} total carbohydrate contents. Physicochemical analysis showed a refractive index of 1.4697-1.4730, specific gravity of 1.369-1.376 g/cm3, peroxide value of 3.33-21.33 meq O2/kg, 184-220 mg/g in saponification number and unsaponifiable matter of 1.1-1.8{\%} in the oil samples. The seeds showed high mineral content such as Ca (2242 mg/kg), K (6393 mg/kg) and Mg (2234 mg/kg). The oil sample from Kelantan, Malaysia contained the lowest saturated fatty acid (SFA) (1.42±0.29{\%}) while Sudan, Yemen contained the highest content of polyunsaturated fatty acid (PUFA) (65.13±5.45{\%}). Monounsaturated fatty acid (MUFA) were found the highest (20.45±2.61{\%}) in the seed samples originated from Iran. Seeds from Iran showed the highest antioxidant activity (IC50 = 1.49 mg/mL) and total phenolic content (30.84 mg GAE/g) while oil sample from Sudan, Yemen has the highest antioxidant activity (IC 50 = 4.48 mg/mL). Seeds from Iran have the highest quality among the seed samples while oil samples from Kelantan, Malaysia was the best among the oil samples in terms of low SFA, high PUFA, MUFA and antioxidant activities.",
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AU - Haron, Hasnah

AU - Grace-Lynn, Chong

AU - Shahar, Suzana

PY - 2014

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N2 - The study was aimed to analyze the physicochemical properties and antioxidant activities in five batches of seeds and oils of Nigella sativa, obtained from Malaysia, Iran and Yemen. Proximate analysis showed that the seeds contained 20.63-28.71% crude fat, 11.35-14.04% crude protein, 5.37-7.93% total moisture, 4.15-4.51% total ash contents and 48.69-57.18% total carbohydrate contents. Physicochemical analysis showed a refractive index of 1.4697-1.4730, specific gravity of 1.369-1.376 g/cm3, peroxide value of 3.33-21.33 meq O2/kg, 184-220 mg/g in saponification number and unsaponifiable matter of 1.1-1.8% in the oil samples. The seeds showed high mineral content such as Ca (2242 mg/kg), K (6393 mg/kg) and Mg (2234 mg/kg). The oil sample from Kelantan, Malaysia contained the lowest saturated fatty acid (SFA) (1.42±0.29%) while Sudan, Yemen contained the highest content of polyunsaturated fatty acid (PUFA) (65.13±5.45%). Monounsaturated fatty acid (MUFA) were found the highest (20.45±2.61%) in the seed samples originated from Iran. Seeds from Iran showed the highest antioxidant activity (IC50 = 1.49 mg/mL) and total phenolic content (30.84 mg GAE/g) while oil sample from Sudan, Yemen has the highest antioxidant activity (IC 50 = 4.48 mg/mL). Seeds from Iran have the highest quality among the seed samples while oil samples from Kelantan, Malaysia was the best among the oil samples in terms of low SFA, high PUFA, MUFA and antioxidant activities.

AB - The study was aimed to analyze the physicochemical properties and antioxidant activities in five batches of seeds and oils of Nigella sativa, obtained from Malaysia, Iran and Yemen. Proximate analysis showed that the seeds contained 20.63-28.71% crude fat, 11.35-14.04% crude protein, 5.37-7.93% total moisture, 4.15-4.51% total ash contents and 48.69-57.18% total carbohydrate contents. Physicochemical analysis showed a refractive index of 1.4697-1.4730, specific gravity of 1.369-1.376 g/cm3, peroxide value of 3.33-21.33 meq O2/kg, 184-220 mg/g in saponification number and unsaponifiable matter of 1.1-1.8% in the oil samples. The seeds showed high mineral content such as Ca (2242 mg/kg), K (6393 mg/kg) and Mg (2234 mg/kg). The oil sample from Kelantan, Malaysia contained the lowest saturated fatty acid (SFA) (1.42±0.29%) while Sudan, Yemen contained the highest content of polyunsaturated fatty acid (PUFA) (65.13±5.45%). Monounsaturated fatty acid (MUFA) were found the highest (20.45±2.61%) in the seed samples originated from Iran. Seeds from Iran showed the highest antioxidant activity (IC50 = 1.49 mg/mL) and total phenolic content (30.84 mg GAE/g) while oil sample from Sudan, Yemen has the highest antioxidant activity (IC 50 = 4.48 mg/mL). Seeds from Iran have the highest quality among the seed samples while oil samples from Kelantan, Malaysia was the best among the oil samples in terms of low SFA, high PUFA, MUFA and antioxidant activities.

KW - Antioxidants

KW - Nigella sativa

KW - Oil

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VL - 43

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