Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia

Shu San Loo, Lik Sin Lim, Nurul Ain Efendi, Damer P. Blake, Shin Ichiro Kawazu, Kiew Lian Wan

Research output: Contribution to journalArticle

Abstract

Coccidiosis, caused by the Eimeria species, greatly affects the poultry industry. Severity of the disease varies depending on the identity of the infecting parasites, encouraging identification of Eimeria species circulating on a farm as a valuable component of chicken management. Conventional methods of Eimeria species identification are time consuming and can be subjective in nature. Given these limitations, molecular approaches have been developed for specific detection of Eimeria species. In this study, faecal samples were collected from commercial broiler farms and subjected to microscopic examination for Eimeria occurrence. Eimeria species were putatively identified by morphological characterisation and grouped into three categories based on oocyst size. Molecular detection of Eimeria species occurrence in these samples was then performed using two published PCR assays (the individual components of a SCAR-based multiplex PCR, and assays developed for quantitative PCR, termed PCR-SCAR 1 and PCR-SCAR 2 here) and a LAMP assay. Comparison of the results obtained demonstrated that the three molecular methods were capable of detecting all Eimeria species of the reference Houghton strain, but showed varying efficiencies in detecting Malaysian field isolates. PCR-SCAR 2 was found to be the most effective, detecting all seven Eimeria species and indicating the presence of Eimeria parasites in most flocks. Differences in the ability of the molecular methods to detect Eimeria may be a consequence of sequence divergence between isolates from different regions, implying that development of region-specific methods using local Eimeria strains may be required to improve the efficiency of molecular assays for Eimeria detection.

Original languageEnglish
Pages (from-to)1425-1432
Number of pages8
JournalSains Malaysiana
Volume48
Issue number7
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Eimeria
Malaysia
chickens
methodology
assays
parasites
farms
poultry industry
coccidiosis
oocysts
disease severity
flocks
quantitative polymerase chain reaction
broiler chickens

Keywords

  • Coccidiosis
  • LAMP
  • Protozoan parasite
  • SCAR

ASJC Scopus subject areas

  • General

Cite this

Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia. / Loo, Shu San; Lim, Lik Sin; Efendi, Nurul Ain; Blake, Damer P.; Kawazu, Shin Ichiro; Wan, Kiew Lian.

In: Sains Malaysiana, Vol. 48, No. 7, 01.01.2019, p. 1425-1432.

Research output: Contribution to journalArticle

Loo, Shu San ; Lim, Lik Sin ; Efendi, Nurul Ain ; Blake, Damer P. ; Kawazu, Shin Ichiro ; Wan, Kiew Lian. / Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia. In: Sains Malaysiana. 2019 ; Vol. 48, No. 7. pp. 1425-1432.
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