Comparison of immunodulatory properties of dental pulp stem cells derived from healthy and inflamed teeth

Farinawati Yazid, Nareshwaran Gnanasegaran, Wijenthiran Kunasekaran, Vijayendran Govindasamy, Sabri Musa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives: The aim of this study was to investigate the immunodulatory properties of dental pulp stem cells derived from healthy (SCD) and inflamed pulp deciduous (SCDIP) tissues. The overall hypothesis is that SCDIP possess equal immune properties with SCD and could be used as an alternative tissue source in regenerative medicine.

Materials and methods: An intra-oral examination was carried out to assess the status of the pulp tissues and group them according to healthy or inflamed. Primary cells were established from these groups, and basic mesenchymal stem cells (MSC) characterizations were conducted. The expression of human leukocyte antigen (HLA), namely HLA-G, HLA-DR, and HLA-ABC were examined in both cell lines using flow cytometry. We further compared the immunosuppressive effects of SCD and SCDIP on phytohemagglutinin-induced T cell proliferation. Supernatants were tested for cytokine profiling using multiplex array.

Results: While SCD exhibited typical MSC characteristics, SCDIP on the other hand, did not. Compared with SCDIP, SCD effectively suppresses mitogen-induced T cells proliferation in a dose-dependent manner, as well as express a higher percentage of HLA-ABC and HLA-G. In addition, levels of several cytokines, such as TNF-α, TNF-β, and IL-2, were drastically suppressed in SCD than SCDIP. Furthermore, a high level of IL-10, an important anti-inflammatory cytokine, was present in SCD compared with SCDIP.

Conclusions: These findings suggest that SCDIP is highly dysfunctional in terms of their stemness and immunomodulatory properties.

Clinical relevance: SCDIP is not a viable therapeutic cell source especially when used in graft versus host disease (GvHD) and organ rejection.

Original languageEnglish
Pages (from-to)2103-2112
Number of pages10
JournalClinical Oral Investigations
Volume18
Issue number9
DOIs
Publication statusPublished - 16 Nov 2014
Externally publishedYes

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Dental Pulp
HLA Antigens
Tooth
Stem Cells
Cytokines
Mesenchymal Stromal Cells
Cell Proliferation
T-Lymphocytes
Oral Diagnosis
Regenerative Medicine
Phytohemagglutinins
Graft vs Host Disease
Immunosuppressive Agents
Mitogens
Interleukin-10
Interleukin-2
Flow Cytometry
Anti-Inflammatory Agents
Cell Line

Keywords

  • Cells
  • Cytokines
  • Deciduous teeth
  • Immunogenicity
  • Regenerative medicine

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Comparison of immunodulatory properties of dental pulp stem cells derived from healthy and inflamed teeth. / Yazid, Farinawati; Gnanasegaran, Nareshwaran; Kunasekaran, Wijenthiran; Govindasamy, Vijayendran; Musa, Sabri.

In: Clinical Oral Investigations, Vol. 18, No. 9, 16.11.2014, p. 2103-2112.

Research output: Contribution to journalArticle

Yazid, Farinawati ; Gnanasegaran, Nareshwaran ; Kunasekaran, Wijenthiran ; Govindasamy, Vijayendran ; Musa, Sabri. / Comparison of immunodulatory properties of dental pulp stem cells derived from healthy and inflamed teeth. In: Clinical Oral Investigations. 2014 ; Vol. 18, No. 9. pp. 2103-2112.
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AU - Yazid, Farinawati

AU - Gnanasegaran, Nareshwaran

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AU - Govindasamy, Vijayendran

AU - Musa, Sabri

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N2 - Objectives: The aim of this study was to investigate the immunodulatory properties of dental pulp stem cells derived from healthy (SCD) and inflamed pulp deciduous (SCDIP) tissues. The overall hypothesis is that SCDIP possess equal immune properties with SCD and could be used as an alternative tissue source in regenerative medicine.Materials and methods: An intra-oral examination was carried out to assess the status of the pulp tissues and group them according to healthy or inflamed. Primary cells were established from these groups, and basic mesenchymal stem cells (MSC) characterizations were conducted. The expression of human leukocyte antigen (HLA), namely HLA-G, HLA-DR, and HLA-ABC were examined in both cell lines using flow cytometry. We further compared the immunosuppressive effects of SCD and SCDIP on phytohemagglutinin-induced T cell proliferation. Supernatants were tested for cytokine profiling using multiplex array.Results: While SCD exhibited typical MSC characteristics, SCDIP on the other hand, did not. Compared with SCDIP, SCD effectively suppresses mitogen-induced T cells proliferation in a dose-dependent manner, as well as express a higher percentage of HLA-ABC and HLA-G. In addition, levels of several cytokines, such as TNF-α, TNF-β, and IL-2, were drastically suppressed in SCD than SCDIP. Furthermore, a high level of IL-10, an important anti-inflammatory cytokine, was present in SCD compared with SCDIP.Conclusions: These findings suggest that SCDIP is highly dysfunctional in terms of their stemness and immunomodulatory properties.Clinical relevance: SCDIP is not a viable therapeutic cell source especially when used in graft versus host disease (GvHD) and organ rejection.

AB - Objectives: The aim of this study was to investigate the immunodulatory properties of dental pulp stem cells derived from healthy (SCD) and inflamed pulp deciduous (SCDIP) tissues. The overall hypothesis is that SCDIP possess equal immune properties with SCD and could be used as an alternative tissue source in regenerative medicine.Materials and methods: An intra-oral examination was carried out to assess the status of the pulp tissues and group them according to healthy or inflamed. Primary cells were established from these groups, and basic mesenchymal stem cells (MSC) characterizations were conducted. The expression of human leukocyte antigen (HLA), namely HLA-G, HLA-DR, and HLA-ABC were examined in both cell lines using flow cytometry. We further compared the immunosuppressive effects of SCD and SCDIP on phytohemagglutinin-induced T cell proliferation. Supernatants were tested for cytokine profiling using multiplex array.Results: While SCD exhibited typical MSC characteristics, SCDIP on the other hand, did not. Compared with SCDIP, SCD effectively suppresses mitogen-induced T cells proliferation in a dose-dependent manner, as well as express a higher percentage of HLA-ABC and HLA-G. In addition, levels of several cytokines, such as TNF-α, TNF-β, and IL-2, were drastically suppressed in SCD than SCDIP. Furthermore, a high level of IL-10, an important anti-inflammatory cytokine, was present in SCD compared with SCDIP.Conclusions: These findings suggest that SCDIP is highly dysfunctional in terms of their stemness and immunomodulatory properties.Clinical relevance: SCDIP is not a viable therapeutic cell source especially when used in graft versus host disease (GvHD) and organ rejection.

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