Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate

Nashriq Mohammad Jailani, Asmahani Azira Abdu Sani, Roman Fischer, Honglei Huang, Wan Mohd Aizat Wan Kamaruddin, Sheila Nathan, Holger B. Kramer, Benedikt M. Kessler, Muhammad Mukram Mohamed Mackeen

Research output: Contribution to journalArticle

Abstract

Using Saccharomyces cerevisiae lysate, two in-solution trypsin digestions (chloroform-methanol-water precipitation and RapiGest) were compared to the recently reported gel-aided sample preparation (GASP) workflow. Our proteomic results showed that GASP afforded the highest number of overall protein identifications and peptide spectrum matches without systematic bias towards peptide or protein size.

Original languageEnglish
Pages (from-to)579-584
Number of pages6
JournalMalaysian Journal of Microbiology
Volume14
Issue numberSpecialissue6
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Workflow
Proteomics
Saccharomyces cerevisiae
Digestion
Gels
Peptides
Chloroform
Trypsin
Methanol
Proteins
Water

Keywords

  • GASP
  • In-solution digestion
  • Proteomic analysis
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate. / Jailani, Nashriq Mohammad; Abdu Sani, Asmahani Azira; Fischer, Roman; Huang, Honglei; Wan Kamaruddin, Wan Mohd Aizat; Nathan, Sheila; Kramer, Holger B.; Kessler, Benedikt M.; Mohamed Mackeen, Muhammad Mukram.

In: Malaysian Journal of Microbiology, Vol. 14, No. Specialissue6, 01.01.2018, p. 579-584.

Research output: Contribution to journalArticle

Jailani, Nashriq Mohammad ; Abdu Sani, Asmahani Azira ; Fischer, Roman ; Huang, Honglei ; Wan Kamaruddin, Wan Mohd Aizat ; Nathan, Sheila ; Kramer, Holger B. ; Kessler, Benedikt M. ; Mohamed Mackeen, Muhammad Mukram. / Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate. In: Malaysian Journal of Microbiology. 2018 ; Vol. 14, No. Specialissue6. pp. 579-584.
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