Comparison of DR. HPV Chip Kit with hybrid capture II assay for the detection of human papillomavirus in clinical samples

a preliminary study.

Saini Rajan, Tae H. Shen, Jacinta Santhanam, Nor Hayati Othman, Norodiyah Othman, Tang Thean Hock

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human papillomavirus (HPV) is well known as an etiological factor for the development of anogenital carcinomas. The aim of our study was to compare the performance of USFDA approved Hybrid II (HCII) Assay and recently introduced DR. HPV Chip Kit for the detection of HPV DNA in clinical cervical scrapings from 40 patients. HPV DNA testing was performed using the automated HCII Assay system and DR. HPV Chip Kit. Taking cytological results as gold standard, it was found that HCII was more sensitive (36.4%) than DR. HPV Chip Kit (18.2%) although specificity was 100% with the latter method. In addition, both these molecular methods had comparable negative and positive predictive values. It was concluded that both HCII and DR. HPV Chip Kit have comparable specificity. However, sensitivity for detection of HPV in clinical samples with HCII is almost double as compared to DR. HPV Chip Kit.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalTropical Biomedicine
Volume24
Issue number1
Publication statusPublished - Jun 2007
Externally publishedYes

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DNA
United States Food and Drug Administration
Carcinoma

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Comparison of DR. HPV Chip Kit with hybrid capture II assay for the detection of human papillomavirus in clinical samples : a preliminary study. / Rajan, Saini; Shen, Tae H.; Santhanam, Jacinta; Othman, Nor Hayati; Othman, Norodiyah; Hock, Tang Thean.

In: Tropical Biomedicine, Vol. 24, No. 1, 06.2007, p. 17-22.

Research output: Contribution to journalArticle

Rajan, Saini ; Shen, Tae H. ; Santhanam, Jacinta ; Othman, Nor Hayati ; Othman, Norodiyah ; Hock, Tang Thean. / Comparison of DR. HPV Chip Kit with hybrid capture II assay for the detection of human papillomavirus in clinical samples : a preliminary study. In: Tropical Biomedicine. 2007 ; Vol. 24, No. 1. pp. 17-22.
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