Comparison of direct examination and in vitro culture for the detection of blastocystis sp. In orang asli stool samples

Samseh Abdullah Noradilah, Norhayati Moktar, Ii Li Lee, Fatmah Md Salleh, Tengku Shahrul Anuar

Research output: Contribution to journalArticle

Abstract

Microscopy-based technique has been widely used in the detection of Blastocystis sp. This study was conducted to compare the techniques used for screening of Blastocystis sp., namely in vitro cultivation of stool specimens in Jones’medium (IVC) followed by Wheatley Trichrome staining and direct examination of stool samples preserved with polyvinyl alcohol (PVA) followed by Wheatley Trichrome staining with single-round polymerase chain reaction (PCR) as the reference technique. The study was performed on 466 stool samples obtained from the aboriginal community in Pahang, Malaysia. IVC showed higher detection rate of Blastocystis sp. (35.6%) than PVA (20.0%). Single-round PCR detected Blastocystis sp. in 41.0% of the stool specimens. The sensitivity and specificity of PVA and IVC in comparison to the reference technique were 75.3% (95% CI: 65.2-83.6) and 68.5% (CI: 63.7-73.3) and 88.6% (CI: 82.7-93.0) and 86.3% (CI: 81.9-90.0), respectively. The agreement between the reference technique and PVA showed statistically significant fair agreement by Cohen Kappa statistics of (K=0.318, p<0.001), meanwhile statistically significant substantial agreement was observed between PCR and IVC by Cohen Kappa (K=0.727, p<0.001). Therefore, in vitro cultivation in Jones’ medium followed by Wheatley Trichrome staining of stool specimens should be used as a screening technique in the detection of Blastocystis sp. infections.

Original languageEnglish
Pages (from-to)2099-2104
Number of pages6
JournalSains Malaysiana
Volume47
Issue number9
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Fingerprint

Blastocystis
Polyvinyl Alcohol
Staining and Labeling
Polymerase Chain Reaction
Malaysia
Microscopy
In Vitro Techniques
Sensitivity and Specificity
Infection

Keywords

  • Blastocystis detection
  • In vitro cultivation
  • Microscopy
  • Polymerase chain reaction
  • Polyvinyl alcohol

ASJC Scopus subject areas

  • General

Cite this

Comparison of direct examination and in vitro culture for the detection of blastocystis sp. In orang asli stool samples. / Noradilah, Samseh Abdullah; Moktar, Norhayati; Lee, Ii Li; Salleh, Fatmah Md; Anuar, Tengku Shahrul.

In: Sains Malaysiana, Vol. 47, No. 9, 01.01.2018, p. 2099-2104.

Research output: Contribution to journalArticle

Noradilah, Samseh Abdullah ; Moktar, Norhayati ; Lee, Ii Li ; Salleh, Fatmah Md ; Anuar, Tengku Shahrul. / Comparison of direct examination and in vitro culture for the detection of blastocystis sp. In orang asli stool samples. In: Sains Malaysiana. 2018 ; Vol. 47, No. 9. pp. 2099-2104.
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