Comparison of conventional versus real-time PCR detection of Brugia malayi DNA from dried blood spots from school children in a low endemic area

N. Rahmah, O. Nurulhasanah, S. Norhayati, Zulkarnain Md Idris, M. Norizan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Microscopic detection of active phase of lymphatic filariasis is indicated by the presence of microfilaria in whole blood. This method is not sensitive and requires relatively large amount of blood sample. PCR allows very sensitive detection of the parasite DNA using a smaller amount of blood; and the use of dried blood spots facilitates sample transportation. Nevertheless, limited studies have been reported on PCR using dried blood spot for detection of Brugia malayi. In this study, we investigated the effects of concentrating whole blood genomic DNA sample and the amplification methods [conventional PCR (C-PCR) and real-time PCR] on the detection of B. malayi DNA from dried blood spots from a very low endemic area in Malaysia. Both C-PCR and real-time PCR detected 2 out of 18 (11%) samples as positive from non-concentrated genomic DNA preparations. After the DNA samples were pooled and concentrated, both C-PCR and real-time PCR detected B. malayi DNA amplifications in 7 out of 18 (39%) samples. However one sample which showed faint band in C-PCR was detected as highly positive in real-time PCR. In conclusion, both C-PCR and real-time PCR using dried blood spots from a low endemic area demonstrated equal sensitivity for detection of B. malayi DNA.

Original languageEnglish
Pages (from-to)54-59
Number of pages6
JournalTropical Biomedicine
Volume27
Issue number1
Publication statusPublished - Apr 2010
Externally publishedYes

Fingerprint

Brugia malayi
Real-Time Polymerase Chain Reaction
DNA
Polymerase Chain Reaction
Filarial Elephantiasis
Microfilariae
Malaysia
Parasites

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Comparison of conventional versus real-time PCR detection of Brugia malayi DNA from dried blood spots from school children in a low endemic area. / Rahmah, N.; Nurulhasanah, O.; Norhayati, S.; Md Idris, Zulkarnain; Norizan, M.

In: Tropical Biomedicine, Vol. 27, No. 1, 04.2010, p. 54-59.

Research output: Contribution to journalArticle

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