Comparison of chitosan scaffold and chitosan-collagen scaffold

a preliminary study.

S. A. Norazril, B. S. Aminuddin, M. M. Norhayati, Mazlyzam Abdul Latif, O. Fauziah, Ruszymah Idrus

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1% acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.

Original languageEnglish
Pages (from-to)186-187
Number of pages2
JournalThe Medical journal of Malaysia
Volume59 Suppl B
Publication statusPublished - May 2004

Fingerprint

Chitosan
Collagen
Tissue Engineering
Tissue Scaffolds
Glycosaminoglycans
Acetic Acid
Polymers
Fibroblasts
Electrons
Skin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Norazril, S. A., Aminuddin, B. S., Norhayati, M. M., Abdul Latif, M., Fauziah, O., & Idrus, R. (2004). Comparison of chitosan scaffold and chitosan-collagen scaffold: a preliminary study. The Medical journal of Malaysia, 59 Suppl B, 186-187.

Comparison of chitosan scaffold and chitosan-collagen scaffold : a preliminary study. / Norazril, S. A.; Aminuddin, B. S.; Norhayati, M. M.; Abdul Latif, Mazlyzam; Fauziah, O.; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 186-187.

Research output: Contribution to journalArticle

Norazril, SA, Aminuddin, BS, Norhayati, MM, Abdul Latif, M, Fauziah, O & Idrus, R 2004, 'Comparison of chitosan scaffold and chitosan-collagen scaffold: a preliminary study.', The Medical journal of Malaysia, vol. 59 Suppl B, pp. 186-187.
Norazril, S. A. ; Aminuddin, B. S. ; Norhayati, M. M. ; Abdul Latif, Mazlyzam ; Fauziah, O. ; Idrus, Ruszymah. / Comparison of chitosan scaffold and chitosan-collagen scaffold : a preliminary study. In: The Medical journal of Malaysia. 2004 ; Vol. 59 Suppl B. pp. 186-187.
@article{7fa271bf675844c5a8abece7c814be46,
title = "Comparison of chitosan scaffold and chitosan-collagen scaffold: a preliminary study.",
abstract = "Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1{\%} acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.",
author = "Norazril, {S. A.} and Aminuddin, {B. S.} and Norhayati, {M. M.} and {Abdul Latif}, Mazlyzam and O. Fauziah and Ruszymah Idrus",
year = "2004",
month = "5",
language = "English",
volume = "59 Suppl B",
pages = "186--187",
journal = "Medical Journal of Malaysia",
issn = "0300-5283",
publisher = "Malaysian Medical Association",

}

TY - JOUR

T1 - Comparison of chitosan scaffold and chitosan-collagen scaffold

T2 - a preliminary study.

AU - Norazril, S. A.

AU - Aminuddin, B. S.

AU - Norhayati, M. M.

AU - Abdul Latif, Mazlyzam

AU - Fauziah, O.

AU - Idrus, Ruszymah

PY - 2004/5

Y1 - 2004/5

N2 - Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1% acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.

AB - Chitosan has similar structure to glycosaminoglycans in the tissue, thus may be a good candidates as tissue engineering scaffold. However, to improve their cell attachment ability, we try to incorporate this natural polymer with collagen by combining it via cross-linking process. In this preliminary study we evaluate the cell attachment ability of chitosan-collagen scaffold versus chitosan scaffold alone. Chitosan and collagen were dissolved in 1% acetic acid and then were frozen for 24 hours before the lyophilizing process. Human skin fibroblasts were seeded into both scaffold and were cultured in F12: DMEM (1:1). Metabolic activity assay were used to evaluate cell attachment ability of scaffold for a period of 1, 3, 7 and 14 days. Scanning electron micrographs shows good cell morphology on chitosan-collagen hybrid scaffold. In conclusion, the incorporation of collagen to chitosan will enhance its cell attachment ability and will be a potential scaffold in tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=16544391429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544391429&partnerID=8YFLogxK

M3 - Article

VL - 59 Suppl B

SP - 186

EP - 187

JO - Medical Journal of Malaysia

JF - Medical Journal of Malaysia

SN - 0300-5283

ER -