Comparison between pork and wild boar meat (Sus scrofa ) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)

Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Safiyyah Shahimi, Norhayati Yaakob, Norrakiah Abdullah Sani, Aminah Abdullah, Abd. Salam Babji, Ma`Aruf Abd. Ghani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A method of PCR-restriction fragment length polymorphism (RFLP) has been utilized to differentiate the mitochondrial genes of pork and wild boar meat (Sus scrofa). The amplification PCR products of 359 bp and 531 bp were successfully amplified from the cyt b gene of these two meats. The amplification product of pork and wild boar using mt-12S rRNA gene successfully produced a single band with molecular size of 456 bp. Three restriction endonucleases (AluI, HindIII and BsaJI) were used to restrict the amplification products of the mitochondrial genes. The restriction enzymes of AluI and BsaJI were identified as potential restriction endonucleases to differentiate those meats. HindIII enzyme was unable to restrict the PCR product of both meats. The genetic differences within the cyt b gene among the two meats were successfully confirmed by PCR-RFLP analysis.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalSains Malaysiana
Volume41
Issue number2
Publication statusPublished - Feb 2012

Fingerprint

Sus scrofa
wild boars
pork
restriction fragment length polymorphism
polymerase chain reaction
meat
restriction endonucleases
genes
enzymes
meat products
ribosomal RNA

Keywords

  • Polymerase chain reaction-restriction fragment length polymorphism
  • Pork
  • Wild boar meat (Sus scrofa)

ASJC Scopus subject areas

  • General

Cite this

Comparison between pork and wild boar meat (Sus scrofa ) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). / Abd. Mutalib, Sahilah; Nazri, Wan Sakeenah Wan; Shahimi, Safiyyah; Yaakob, Norhayati; Abdullah Sani, Norrakiah; Abdullah, Aminah; Babji, Abd. Salam; Abd. Ghani, Ma`Aruf.

In: Sains Malaysiana, Vol. 41, No. 2, 02.2012, p. 199-204.

Research output: Contribution to journalArticle

Abd. Mutalib, Sahilah ; Nazri, Wan Sakeenah Wan ; Shahimi, Safiyyah ; Yaakob, Norhayati ; Abdullah Sani, Norrakiah ; Abdullah, Aminah ; Babji, Abd. Salam ; Abd. Ghani, Ma`Aruf. / Comparison between pork and wild boar meat (Sus scrofa ) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In: Sains Malaysiana. 2012 ; Vol. 41, No. 2. pp. 199-204.
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