Comparison between mt-DNA D-loop and Cyt B primers for porcine DNA detection in meat products

Azhana Hamzah, Sahilah Abd. Mutalib, Abd. Salam Babji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study was conducted to detect the presence of porcine DNA in meat products in the market using conventional polymerase chain reaction (PCR) and commercial PCR-southern hybridization analysis. Porcine DNA detection in meat products was tested due to some issues associated with the adulteration of food products in Malaysia. This is an important issue especially for Halal authentication which is required for some religious practices such as in Islam and Hinduisms. Many techniques have been developed for determining the Halal status of food products. In this paper, mt-DNA D-loop primer and cytochrome (cyt) b were used to detect the presence of porcine DNA in meat products. Positive and negative controls were always present for each batch of extraction. DNA of raw pork meat was used as a positive control while nucleus free water is used as negative control. A pair of oligonucleotide primer was used namely Pork1 and Pork2 which produced amplicon of 531 base pair (bp) in size. While, PCR-southern hybridization was conducted using primers readily supplied by commercial PCR-Southern hybridization and produced amplicon with 276 bp in size. In the present study, demonstrated that none of the samples were contaminated with porcine residuals but selected samples with pork meat were positive. The species-specific PCR amplification yielded excellent results for identification of pork derivatives in food products and it is a potentially reliable and suitable technique in routine food analysis for Halal certification.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages669-673
Number of pages5
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

primers
polymerase chain reaction
deoxyribonucleic acid
food
products
Malaysia
certification
oligonucleotides
cytochromes
nuclei
water

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Comparison between mt-DNA D-loop and Cyt B primers for porcine DNA detection in meat products. / Hamzah, Azhana; Abd. Mutalib, Sahilah; Babji, Abd. Salam.

AIP Conference Proceedings. Vol. 1571 2013. p. 669-673.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hamzah, A, Abd. Mutalib, S & Babji, AS 2013, Comparison between mt-DNA D-loop and Cyt B primers for porcine DNA detection in meat products. in AIP Conference Proceedings. vol. 1571, pp. 669-673, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858731
Hamzah, Azhana ; Abd. Mutalib, Sahilah ; Babji, Abd. Salam. / Comparison between mt-DNA D-loop and Cyt B primers for porcine DNA detection in meat products. AIP Conference Proceedings. Vol. 1571 2013. pp. 669-673
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