Abstract
Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.
| Original language | English |
|---|---|
| Pages (from-to) | 734-737 |
| Number of pages | 4 |
| Journal | Singapore Medical Journal |
| Volume | 52 |
| Issue number | 10 |
| Publication status | Published - Oct 2011 |
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Keywords
- Liquid nitrogen
- Sperm cryopreservation
- Ultra-low temperature
ASJC Scopus subject areas
- Medicine(all)
Cite this
Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia. / Abd Rahman, Rahana; Ng, S. P.; Chooi Fun, Leong; Rahimah, M. D.
In: Singapore Medical Journal, Vol. 52, No. 10, 10.2011, p. 734-737.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia
AU - Abd Rahman, Rahana
AU - Ng, S. P.
AU - Chooi Fun, Leong
AU - Rahimah, M. D.
PY - 2011/10
Y1 - 2011/10
N2 - Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.
AB - Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.
KW - Liquid nitrogen
KW - Sperm cryopreservation
KW - Ultra-low temperature
UR - http://www.scopus.com/inward/record.url?scp=80054774294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80054774294&partnerID=8YFLogxK
M3 - Article
C2 - 22009393
AN - SCOPUS:80054774294
VL - 52
SP - 734
EP - 737
JO - Singapore Medical Journal
JF - Singapore Medical Journal
SN - 0037-5675
IS - 10
ER -