Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia

Rahana Abd Rahman, S. P. Ng, Leong Chooi Fun, M. D. Rahimah

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.

Original languageEnglish
Pages (from-to)734-737
Number of pages4
JournalSingapore Medical Journal
Volume52
Issue number10
Publication statusPublished - Oct 2011

Fingerprint

Cryopreservation
Freezing
Nitrogen
Semen
Spermatozoa
Hospital Obstetrics and Gynecology Department
Temperature
DNA
Malaysia
Gynecology
Prospective Studies

Keywords

  • Liquid nitrogen
  • Sperm cryopreservation
  • Ultra-low temperature

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia. / Abd Rahman, Rahana; Ng, S. P.; Chooi Fun, Leong; Rahimah, M. D.

In: Singapore Medical Journal, Vol. 52, No. 10, 10.2011, p. 734-737.

Research output: Contribution to journalArticle

@article{f8894d500e0245c4be1c5087704baa1e,
title = "Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia",
abstract = "Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.",
keywords = "Liquid nitrogen, Sperm cryopreservation, Ultra-low temperature",
author = "{Abd Rahman}, Rahana and Ng, {S. P.} and {Chooi Fun}, Leong and Rahimah, {M. D.}",
year = "2011",
month = "10",
language = "English",
volume = "52",
pages = "734--737",
journal = "Singapore Medical Journal",
issn = "0037-5675",
publisher = "Singapore Medical Association",
number = "10",

}

TY - JOUR

T1 - Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia

AU - Abd Rahman, Rahana

AU - Ng, S. P.

AU - Chooi Fun, Leong

AU - Rahimah, M. D.

PY - 2011/10

Y1 - 2011/10

N2 - Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.

AB - Introduction: This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. Methods: This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Results: Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. Conclusion: This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.

KW - Liquid nitrogen

KW - Sperm cryopreservation

KW - Ultra-low temperature

UR - http://www.scopus.com/inward/record.url?scp=80054774294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054774294&partnerID=8YFLogxK

M3 - Article

VL - 52

SP - 734

EP - 737

JO - Singapore Medical Journal

JF - Singapore Medical Journal

SN - 0037-5675

IS - 10

ER -