Comparative proteomics analysis of oral cancer cell lines: Identification of cancer associated proteins

Saiful A. Karsani, Nor A. Saihen, Rosnah B. Zain, Sok Ching Cheong, Mariati Abdul Rahman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: A limiting factor in performing proteomics analysis on cancerous cells is the difficulty in obtaining sufficient amounts of starting material. Cell lines can be used as a simplified model system for studying changes that accompany tumorigenesis. This study used two-dimensional gel electrophoresis (2DE) to compare the whole cell proteome of oral cancer cell lines vs normal cells in an attempt to identify cancer associated proteins. Results: Three primary cell cultures of normal cells with a limited lifespan without hTERT immortalization have been successfully established. 2DE was used to compare the whole cell proteome of these cells with that of three oral cancer cell lines. Twenty four protein spots were found to have changed in abundance. MALDI TOF/TOF was then used to determine the identity of these proteins. Identified proteins were classified into seven functional categories - structural proteins, enzymes, regulatory proteins, chaperones and others. IPA core analysis predicted that 18 proteins were related to cancer with involvements in hyperplasia, metastasis, invasion, growth and tumorigenesis. The mRNA expressions of two proteins - 14-3-3 protein sigma and Stress-induced-phosphoprotein 1 - were found to correlate with the corresponding proteins' abundance.Conclusions: The outcome of this analysis demonstrated that a comparative study of whole cell proteome of cancer versus normal cell lines can be used to identify cancer associated proteins.

Original languageEnglish
Article number3
JournalProteome Science
Volume12
Issue number1
DOIs
Publication statusPublished - 15 Jan 2014

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Mouth Neoplasms
Proteomics
Cells
Cell Line
Neoplasms
Proteins
Proteome
14-3-3 Proteins
Carcinogenesis
Core analysis
Primary Cell Culture
Phosphoproteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Cell culture
Hyperplasia
Gels
Neoplasm Metastasis
Messenger RNA

Keywords

  • Cancer associated proteins
  • Cell lines
  • Oral cancer
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Comparative proteomics analysis of oral cancer cell lines : Identification of cancer associated proteins. / Karsani, Saiful A.; Saihen, Nor A.; Zain, Rosnah B.; Cheong, Sok Ching; Abdul Rahman, Mariati.

In: Proteome Science, Vol. 12, No. 1, 3, 15.01.2014.

Research output: Contribution to journalArticle

Karsani, Saiful A. ; Saihen, Nor A. ; Zain, Rosnah B. ; Cheong, Sok Ching ; Abdul Rahman, Mariati. / Comparative proteomics analysis of oral cancer cell lines : Identification of cancer associated proteins. In: Proteome Science. 2014 ; Vol. 12, No. 1.
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