Comparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cells

Abdul Rahman Hayati, Adzman-Sellymiah, Chua Kien Hui, Raman Nur Fatin Aqilah, Geok Chin Tan, Simat Siti Fatimah, Yun Hsuen Lim, Rahim Nur Syahrina, Mohd Manzor Nur Fariha

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells population has been successfully isolated from the matrix and characterised for its stemness properties. Endothelial cells from the vein of the umbilical cord which are usually termed as HUVEC are also of interest to researchers as it can be manipulated in vitro to study endothelial cells behaviour and vascular-associated diseases. Immunophenotyping for positive and negative markers of mesenchymal stem cells was performed on HUVEC and compared to the closest stem cells that were isolated from the umbilical cord matrix which is termed as HUCMSC in this study. The expressions of a set of stemness genes were also detected using qPCR. The results showed that HUVEC strongly expressed hematopoietic and mesenchymal stem cells markers (CD31, CD34, CD9, CD44, CD73 and CD90) while HUCMSC possess mesenchymal stem cells properties with strong expression of CD9, CD44, CD73 and CD90. Interestingly, the expression of stemness genes is detected higher in HUVEC compared to HUCMSC with possibilities of having neural properties as evidenced by the high expression of FGF4, FZD9, Nestin and SOX2. Thus, it is suggested that HUVEC exhibited some stem cells characteristics and could be further investigated for possible application in research as well as in future cells therapy.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalInternational Journal of Research in Pharmaceutical Sciences
Volume9
Issue numberSpecial Issue 2
DOIs
Publication statusPublished - 28 Dec 2018

Fingerprint

Umbilical Cord
Human Umbilical Vein Endothelial Cells
Mesenchymal Stromal Cells
Stem Cells
Endothelial Cells
Nestin
Immunophenotyping
Cell- and Tissue-Based Therapy
Hematopoietic Stem Cells
Vascular Diseases
Veins
Research Personnel
Gene Expression
Research
Population
Genes

Keywords

  • Cell differentiation
  • Flow cytometry
  • Ribonucleic acid (RNA)
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Comparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cells. / Hayati, Abdul Rahman; Adzman-Sellymiah, ; Kien Hui, Chua; Aqilah, Raman Nur Fatin; Tan, Geok Chin; Fatimah, Simat Siti; Lim, Yun Hsuen; Syahrina, Rahim Nur; Fariha, Mohd Manzor Nur.

In: International Journal of Research in Pharmaceutical Sciences, Vol. 9, No. Special Issue 2, 28.12.2018, p. 51-59.

Research output: Contribution to journalArticle

Hayati, Abdul Rahman ; Adzman-Sellymiah, ; Kien Hui, Chua ; Aqilah, Raman Nur Fatin ; Tan, Geok Chin ; Fatimah, Simat Siti ; Lim, Yun Hsuen ; Syahrina, Rahim Nur ; Fariha, Mohd Manzor Nur. / Comparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cells. In: International Journal of Research in Pharmaceutical Sciences. 2018 ; Vol. 9, No. Special Issue 2. pp. 51-59.
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