Comparative efficacy sensitivity and specificity of the tests used for the diagnosis of Helicobacter pylori

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Abstract

A total number of 157 samples were examined by 4 different tests-In-house rapid urease (iRUT), Culture, Histopathology and Immunochromatography (Immuno CardSTAT) for the detection of Helicobacter pylori from the patients reported to Hospital Kuala Lumpur, Malaysia during 2007 to 2008. Out of the samples examined 47 (29.9%) were positive for//, pylori by the tests used in the laboratory. Efficacy of detection of the bacteria by the tests- In-house rapid urease, Culture, Histopathology and Immuno CardSTAT were 31.8, 13.9, 30.3 and 32.8%, respectively. However, sensitivity and specificity of the iRUT were 91.5 and 93.6%, respectively and the Positive Predictive Value (PPV) was 86% and the Negative Predictive Value (NPV) was 96.3%. The sensitivity for Immuno CardSTAT rapid test was 100% and the specificity was 79.3%. The PPV was 50% and the NPV was 100%. Convenient methods to the authors were 'In house rapid urease test and Immunochromatography though variability of specificities were observed.

Original languageEnglish
Pages (from-to)1057-1061
Number of pages5
JournalPakistan Journal of Biological Sciences
Volume13
Issue number21
Publication statusPublished - 2010

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Helicobacter pylori
urease
immunoaffinity chromatography
histopathology
testing
pylorus
Malaysia
sampling
bacteria

Keywords

  • Helicobacter pylori
  • Histopathology
  • Immunochromatogrphy
  • In house urease test
  • Microaerophilic

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Comparative efficacy sensitivity and specificity of the tests used for the diagnosis of Helicobacter pylori",
abstract = "A total number of 157 samples were examined by 4 different tests-In-house rapid urease (iRUT), Culture, Histopathology and Immunochromatography (Immuno CardSTAT) for the detection of Helicobacter pylori from the patients reported to Hospital Kuala Lumpur, Malaysia during 2007 to 2008. Out of the samples examined 47 (29.9{\%}) were positive for//, pylori by the tests used in the laboratory. Efficacy of detection of the bacteria by the tests- In-house rapid urease, Culture, Histopathology and Immuno CardSTAT were 31.8, 13.9, 30.3 and 32.8{\%}, respectively. However, sensitivity and specificity of the iRUT were 91.5 and 93.6{\%}, respectively and the Positive Predictive Value (PPV) was 86{\%} and the Negative Predictive Value (NPV) was 96.3{\%}. The sensitivity for Immuno CardSTAT rapid test was 100{\%} and the specificity was 79.3{\%}. The PPV was 50{\%} and the NPV was 100{\%}. Convenient methods to the authors were 'In house rapid urease test and Immunochromatography though variability of specificities were observed.",
keywords = "Helicobacter pylori, Histopathology, Immunochromatogrphy, In house urease test, Microaerophilic",
author = "N. Suhaila and Salasawati Hussin and Rahman, {Md. Mostafizur}",
year = "2010",
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AU - Hussin, Salasawati

AU - Rahman, Md. Mostafizur

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AB - A total number of 157 samples were examined by 4 different tests-In-house rapid urease (iRUT), Culture, Histopathology and Immunochromatography (Immuno CardSTAT) for the detection of Helicobacter pylori from the patients reported to Hospital Kuala Lumpur, Malaysia during 2007 to 2008. Out of the samples examined 47 (29.9%) were positive for//, pylori by the tests used in the laboratory. Efficacy of detection of the bacteria by the tests- In-house rapid urease, Culture, Histopathology and Immuno CardSTAT were 31.8, 13.9, 30.3 and 32.8%, respectively. However, sensitivity and specificity of the iRUT were 91.5 and 93.6%, respectively and the Positive Predictive Value (PPV) was 86% and the Negative Predictive Value (NPV) was 96.3%. The sensitivity for Immuno CardSTAT rapid test was 100% and the specificity was 79.3%. The PPV was 50% and the NPV was 100%. Convenient methods to the authors were 'In house rapid urease test and Immunochromatography though variability of specificities were observed.

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