Comparative effects of α-tocopherol and γ-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes

Musalmah Mazlan, Then Sue Mian, Gapor Mat Top, Wan Zurinah Wan Ngah

    Research output: Contribution to journalArticle

    66 Citations (Scopus)

    Abstract

    Oxidative stress is thought to be one of the factors that cause neurodegeneration and that this can be inhibited by antioxidants. Since astrocytes support the survival of central nervous system (CNS) neurons, we compared the effect of α-tocopherol and γ-tocotrienol in minimizing the cytotoxic damage induced by H2O2, a pro-oxidant. Primary astrocyte cultures were pretreated with either α-tocopherol or γ-tocotrienol for 1 h before incubation with 100 μM H2O 2 for 24 h. Cell viability was then assessed using the MTS assay while apoptosis was determined using a commercial ELISA kit as well as by fluorescent staining of live and apoptotic cells. The uptake of α-tocopherol and γ-tocotrienol by astrocytes were also determined using HPLC. Results showed that γ-tocotrienol is toxic at concentrations > 200 μM but protects against H2O2 induced cell loss and apoptosis in a dose dependent manner up to 100 μM. α-Tocopherol was not cytotoxic in the concentration range tested (up to 750 μM), reduced apoptosis to the same degree as that of γ-tocotrienol but was less effective in maintaining the viable cell number. Since the uptake of α-tocopherol and γ-tocotrienol by astrocytes is similar, this may reflect the roles of these 2 vitamin E subfamilies in inhibiting apoptosis and stimulating proliferation in astrocytes.

    Original languageEnglish
    Pages (from-to)5-12
    Number of pages8
    JournalJournal of the Neurological Sciences
    Volume243
    Issue number1-2
    DOIs
    Publication statusPublished - 15 Apr 2006

    Fingerprint

    Tocotrienols
    Tocopherols
    Astrocytes
    Hydrogen Peroxide
    Apoptosis
    Poisons
    Vitamin E
    Reactive Oxygen Species
    Cell Survival
    Oxidative Stress
    Central Nervous System
    Cell Count
    Antioxidants
    Enzyme-Linked Immunosorbent Assay
    High Pressure Liquid Chromatography
    Staining and Labeling
    Neurons

    Keywords

    • Apoptosis
    • Astrocytes
    • Cell viability
    • Hydrogen peroxide
    • Oxidative stress

    ASJC Scopus subject areas

    • Ageing
    • Clinical Neurology
    • Surgery
    • Developmental Neuroscience
    • Neurology
    • Neuroscience(all)

    Cite this

    Comparative effects of α-tocopherol and γ-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes. / Mazlan, Musalmah; Sue Mian, Then; Mat Top, Gapor; Zurinah Wan Ngah, Wan.

    In: Journal of the Neurological Sciences, Vol. 243, No. 1-2, 15.04.2006, p. 5-12.

    Research output: Contribution to journalArticle

    Mazlan, Musalmah ; Sue Mian, Then ; Mat Top, Gapor ; Zurinah Wan Ngah, Wan. / Comparative effects of α-tocopherol and γ-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes. In: Journal of the Neurological Sciences. 2006 ; Vol. 243, No. 1-2. pp. 5-12.
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