Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia malayi from Northeast Borneo and Thailand

Mun Yik Fong, Rahmah Noordin, Yee Ling Lau, Fei Wen Cheong, Muhammad Hafiznur Yunus, Zulkarnain Md Idris

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of B. malayi isolated from cats and humans in Thailand. Multiple alignment analysis revealed that B. malayi ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct B. malayi clusters. The first cluster consisted of Northeast Borneo B. malayi isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that B. malayi in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of B. malayi infection across the region using ITS1-based molecular techniques.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalParasitology
Volume140
Issue number1
DOIs
Publication statusPublished - Jan 2013
Externally publishedYes

Fingerprint

Borneo
Brugia malayi
Thailand
internal transcribed spacers
nucleotide sequences
Molecular Epidemiology
Islands
Filarial Elephantiasis
filariasis
Microfilariae
molecular epidemiology
microfilariae
Helminths
helminths
topology
geographical distribution
Cats
cats

Keywords

  • Borneo Island
  • Brugia malayi
  • genetic variation
  • ITS1
  • Thailand

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia malayi from Northeast Borneo and Thailand. / Fong, Mun Yik; Noordin, Rahmah; Lau, Yee Ling; Cheong, Fei Wen; Yunus, Muhammad Hafiznur; Md Idris, Zulkarnain.

In: Parasitology, Vol. 140, No. 1, 01.2013, p. 39-45.

Research output: Contribution to journalArticle

Fong, Mun Yik ; Noordin, Rahmah ; Lau, Yee Ling ; Cheong, Fei Wen ; Yunus, Muhammad Hafiznur ; Md Idris, Zulkarnain. / Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia malayi from Northeast Borneo and Thailand. In: Parasitology. 2013 ; Vol. 140, No. 1. pp. 39-45.
@article{2b2e476bc8274966b8f0e869180ccfea,
title = "Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia malayi from Northeast Borneo and Thailand",
abstract = "Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of B. malayi isolated from cats and humans in Thailand. Multiple alignment analysis revealed that B. malayi ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct B. malayi clusters. The first cluster consisted of Northeast Borneo B. malayi isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that B. malayi in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of B. malayi infection across the region using ITS1-based molecular techniques.",
keywords = "Borneo Island, Brugia malayi, genetic variation, ITS1, Thailand",
author = "Fong, {Mun Yik} and Rahmah Noordin and Lau, {Yee Ling} and Cheong, {Fei Wen} and Yunus, {Muhammad Hafiznur} and {Md Idris}, Zulkarnain",
year = "2013",
month = "1",
doi = "10.1017/S0031182012001242",
language = "English",
volume = "140",
pages = "39--45",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "1",

}

TY - JOUR

T1 - Comparative analysis of ITS1 nucleotide sequence reveals distinct genetic difference between Brugia malayi from Northeast Borneo and Thailand

AU - Fong, Mun Yik

AU - Noordin, Rahmah

AU - Lau, Yee Ling

AU - Cheong, Fei Wen

AU - Yunus, Muhammad Hafiznur

AU - Md Idris, Zulkarnain

PY - 2013/1

Y1 - 2013/1

N2 - Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of B. malayi isolated from cats and humans in Thailand. Multiple alignment analysis revealed that B. malayi ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct B. malayi clusters. The first cluster consisted of Northeast Borneo B. malayi isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that B. malayi in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of B. malayi infection across the region using ITS1-based molecular techniques.

AB - Brugia malayi is one of the parasitic worms which causes lymphatic filariasis in humans. Its geographical distribution includes a large part of Asia. Despite its wide distribution, very little is known about the genetic variation and molecular epidemiology of this species. In this study, the internal transcribed spacer 1 (ITS1) nucleotide sequences of B. malayi from microfilaria-positive human blood samples in Northeast Borneo Island were determined, and compared with published ITS1 sequences of B. malayi isolated from cats and humans in Thailand. Multiple alignment analysis revealed that B. malayi ITS1 sequences from Northeast Borneo were more similar to each other than to those from Thailand. Phylogenetic trees inferred using Neighbour-Joining and Maximum Parsimony methods showed similar topology, with 2 distinct B. malayi clusters. The first cluster consisted of Northeast Borneo B. malayi isolates, whereas the second consisted of the Thailand isolates. The findings of this study suggest that B. malayi in Borneo Island has diverged significantly from those of mainland Asia, and this has implications for the diagnosis of B. malayi infection across the region using ITS1-based molecular techniques.

KW - Borneo Island

KW - Brugia malayi

KW - genetic variation

KW - ITS1

KW - Thailand

UR - http://www.scopus.com/inward/record.url?scp=84872041119&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872041119&partnerID=8YFLogxK

U2 - 10.1017/S0031182012001242

DO - 10.1017/S0031182012001242

M3 - Article

C2 - 22917270

AN - SCOPUS:84872041119

VL - 140

SP - 39

EP - 45

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 1

ER -