Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis

S. S. Abdul Fattah, R. Mohamed, Jamaliah Md Jahim, R. M. Illias, Farah Diba Abu Bakar, Abdul Munir Abd. Murad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, commercial cellulases and hemicellulases were evaluated for their hydrolytic activity towards pretreated oil palm empty fruit bunches (OPEFB). A total of three commercial cellulase preparations, Novozyme Celluclast®, Novozyme Cellic®Ctec, Dupont Accellerase®1500, and a commercial hemicellulase preparation, Novozyme Cellic®Htec, were evaluated. The assays were performed either using the cellulase alone or cellulase in combination with the hemicellulase, formulated at different enzyme activity ratios. Among the three cellulases, the Novozyme Cellic®Ctec yielded the highest reducing sugars, whereby 32.9% hydrolysis yield of OPEFB was achieved. The addition of the commercial hemicellulase to Celluclast® and Accellerase®1500 enhanced OPEFB hydrolysis. However, the addition of the hemicellulase to Cellic®Ctec, failed to enhance the production of the reducing sugars. Nevertheless, the amount of reducing sugars produced using Cellic®Ctec alone was the highest when compared to other enzyme combinations. It can be concluded that among the three commercial cellulases evaluated, the Novozyme Cellic®Ctec is the best enzyme for OPEFB hydrolysis.

Original languageEnglish
Title of host publication2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1784
ISBN (Electronic)9780735414464
DOIs
Publication statusPublished - 17 Nov 2016
Event2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016 - Selangor, Malaysia
Duration: 13 Apr 201614 Apr 2016

Other

Other2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016
CountryMalaysia
CitySelangor
Period13/4/1614/4/16

Fingerprint

fruits
hydrolysis
sugars
oils
enzymes
preparation
enzyme activity

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Abdul Fattah, S. S., Mohamed, R., Md Jahim, J., Illias, R. M., Abu Bakar, F. D., & Abd. Murad, A. M. (2016). Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium (Vol. 1784). [020002] American Institute of Physics Inc.. https://doi.org/10.1063/1.4966712

Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis. / Abdul Fattah, S. S.; Mohamed, R.; Md Jahim, Jamaliah; Illias, R. M.; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir.

2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016. 020002.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abdul Fattah, SS, Mohamed, R, Md Jahim, J, Illias, RM, Abu Bakar, FD & Abd. Murad, AM 2016, Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis. in 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. vol. 1784, 020002, American Institute of Physics Inc., 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, Selangor, Malaysia, 13/4/16. https://doi.org/10.1063/1.4966712
Abdul Fattah SS, Mohamed R, Md Jahim J, Illias RM, Abu Bakar FD, Abd. Murad AM. Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis. In 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784. American Institute of Physics Inc. 2016. 020002 https://doi.org/10.1063/1.4966712
Abdul Fattah, S. S. ; Mohamed, R. ; Md Jahim, Jamaliah ; Illias, R. M. ; Abu Bakar, Farah Diba ; Abd. Murad, Abdul Munir. / Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis. 2016 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Vol. 1784 American Institute of Physics Inc., 2016.
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