Combined ginger extract & Gelam honey modulate Ras/ERK and PI3K/AKT pathway genes in colon cancer HT29 cells

Analhuda Abdullah Tahir, Nur Fathiah Abdul Sani, Noor Azian Murad, Suzana Makpol, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: The interconnected Ras/ERK and PI3K/AKT pathways play a central role in colorectal tumorigenesis, and they are targets for elucidating mechanisms involved in attempts to induce colon cancer cell death. Both ginger (Zingiber officinale) and honey have been shown to exhibit anti-tumor and anti-inflammation properties against many types of cancer, including colorectal cancer. However, there are currently no reports showing the combined effect of these two dietary compounds in cancer growth inhibition. The aim of this study was to evaluate the synergistic effect of crude ginger extract and Gelam honey in combination as potential cancer chemopreventive agents against the colorectal cancer cell line HT29. Methods: The cells were divided into 4 groups: the first group represents HT29 cells without treatment, the second and third groups were cells treated singly with either ginger or Gelam honey, respectively, and the last group represents cells treated with ginger and Gelam honey combined. Results: The results of MTS assay showed that the IC<inf>50</inf> of ginger and Gelam honey alone were 5.2 mg/ml and 80 mg/ml, respectively, whereas the IC<inf>50</inf> of the combination treatment was 3 mg/ml of ginger plus 27 mg/ml of Gelam honey with a combination index of < 1, suggesting synergism. Cell death in response to the combined ginger and Gelam honey treatment was associated with the stimulation of early apoptosis (upregulation of caspase 9 and IκB genes) accompanied by downregulation of the KRAS, ERK, AKT, Bcl-xL, NFkB (p65) genes in a synergistic manner. Conclusions: In conclusion, the combination of ginger and Gelam honey may be an effective chemopreventive and therapeutic strategy for inducing the death of colon cancer cells.

Original languageEnglish
Article number31
JournalNutrition Journal
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Apr 2015

Fingerprint

Ginger
HT29 Cells
Honey
Phosphatidylinositol 3-Kinases
Colonic Neoplasms
Genes
Inhibitory Concentration 50
Colorectal Neoplasms
Neoplasms
Cell Death
Caspase 9
Complex Mixtures
Carcinogenesis
Up-Regulation
Down-Regulation
Apoptosis
Inflammation
Cell Line

Keywords

  • Gelam honey
  • Ginger
  • HT29 colon cancer cells
  • Ras/ERK and PI3K/AKT pathways

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Combined ginger extract & Gelam honey modulate Ras/ERK and PI3K/AKT pathway genes in colon cancer HT29 cells. / Tahir, Analhuda Abdullah; Sani, Nur Fathiah Abdul; Murad, Noor Azian; Makpol, Suzana; Ngah, Wan Zurinah Wan; Mohd Yusof, Yasmin Anum.

In: Nutrition Journal, Vol. 14, No. 1, 31, 01.04.2015.

Research output: Contribution to journalArticle

Tahir, Analhuda Abdullah ; Sani, Nur Fathiah Abdul ; Murad, Noor Azian ; Makpol, Suzana ; Ngah, Wan Zurinah Wan ; Mohd Yusof, Yasmin Anum. / Combined ginger extract & Gelam honey modulate Ras/ERK and PI3K/AKT pathway genes in colon cancer HT29 cells. In: Nutrition Journal. 2015 ; Vol. 14, No. 1.
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AU - Sani, Nur Fathiah Abdul

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AU - Makpol, Suzana

AU - Ngah, Wan Zurinah Wan

AU - Mohd Yusof, Yasmin Anum

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AB - Background: The interconnected Ras/ERK and PI3K/AKT pathways play a central role in colorectal tumorigenesis, and they are targets for elucidating mechanisms involved in attempts to induce colon cancer cell death. Both ginger (Zingiber officinale) and honey have been shown to exhibit anti-tumor and anti-inflammation properties against many types of cancer, including colorectal cancer. However, there are currently no reports showing the combined effect of these two dietary compounds in cancer growth inhibition. The aim of this study was to evaluate the synergistic effect of crude ginger extract and Gelam honey in combination as potential cancer chemopreventive agents against the colorectal cancer cell line HT29. Methods: The cells were divided into 4 groups: the first group represents HT29 cells without treatment, the second and third groups were cells treated singly with either ginger or Gelam honey, respectively, and the last group represents cells treated with ginger and Gelam honey combined. Results: The results of MTS assay showed that the IC50 of ginger and Gelam honey alone were 5.2 mg/ml and 80 mg/ml, respectively, whereas the IC50 of the combination treatment was 3 mg/ml of ginger plus 27 mg/ml of Gelam honey with a combination index of < 1, suggesting synergism. Cell death in response to the combined ginger and Gelam honey treatment was associated with the stimulation of early apoptosis (upregulation of caspase 9 and IκB genes) accompanied by downregulation of the KRAS, ERK, AKT, Bcl-xL, NFkB (p65) genes in a synergistic manner. Conclusions: In conclusion, the combination of ginger and Gelam honey may be an effective chemopreventive and therapeutic strategy for inducing the death of colon cancer cells.

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